Cadmium-mediated activation of the metal response element in human neuroblastoma cells lacking functional metal response element-binding transcription factor-1

被引:26
作者
Chu, WA
Moehlenkamp, JD
Bittel, D
Andrews, GK
Johnson, JA
机构
[1] Univ Kansas, Med Ctr, Dept Pharmacol, Kansas City, KS 66160 USA
[2] Univ Kansas, Med Ctr, Dept Toxicol, Kansas City, KS 66160 USA
[3] Univ Kansas, Med Ctr, Dept Therapeut, Kansas City, KS 66160 USA
[4] Univ Kansas, Med Ctr, Dept Biochem, Kansas City, KS 66160 USA
[5] Univ Kansas, Med Ctr, Dept Mol Biol, Kansas City, KS 66160 USA
关键词
D O I
10.1074/jbc.274.9.5279
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Metal response element-binding transcription factor-1 (MTF-1) binds specifically to metal response elements (MREs) and transactivates metallothionein (MT) gene expression in response to zinc and cadmium. This investigation contrasts the mechanism of mouse MT gene (mMT I) promoter activation by cadmium and zinc in IMR-32 human neuroblastoma cells to determine whether MTF-1 binding to the MRE is necessary for activation by these metals. Cadmium activated a mMT-1 promoter (-150 base pairs) luciferase reporter 20-25-fold through a MRE-dependent mechanism. In contrast, zinc had little effect on the mMT-1 luciferase reporter, IMR-32 cells lacked MRE binding activity, and treatment with zinc in vitro or in vivo did not generate a MTF-1 MRE complex, suggesting that IMR-32 cells lack functional MTF-1 Overexpression of mMTF-1 regenerated a zinc-mediated induction of the MRE without affecting cadmium activation. Because no other transition metals tested activated the MRE, this effect appeared to be cadmium-specific. These data demonstrate that in IMR-32 human neuroblastoma cells, zinc and cadmium can use independent mechanisms for activation of the mMT-I promoter and cadmium-mediated MRE activation is independent of TTF-l and zinc.
引用
收藏
页码:5279 / 5284
页数:6
相关论文
共 39 条
[21]   ENHANCED SENSITIVITY TO OXIDATIVE STRESS IN CULTURED EMBRYONIC-CELLS FROM TRANSGENIC MICE DEFICIENT IN METALLOTHIONEIN-I AND METALLOTHIONEIN-II GENES [J].
LAZO, JS ;
KONDO, Y ;
DELLAPIAZZA, D ;
MICHALSKA, AE ;
CHOO, KHA ;
PITT, BR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (10) :5506-5510
[22]   Identification of a signal transducer and activator of transcription (STAT) binding site in the mouse metallothionein-I promoter involved in interleukin-6-induced gene expression [J].
Lee, DK ;
Carrasco, J ;
Hidalgo, J ;
Andrews, GK .
BIOCHEMICAL JOURNAL, 1999, 337 :59-65
[23]   Participation of upstream stimulator factor (USF) in cadmium-induction of the mouse metallothionein-I gene [J].
Li, QW ;
Hu, NM ;
Daggett, MAF ;
Chu, WA ;
Bittel, D ;
Johnson, JA ;
Andrews, GK .
NUCLEIC ACIDS RESEARCH, 1998, 26 (22) :5182-5189
[24]   TARGETED DISRUPTION OF METALLOTHIONEIN-I AND METALLOTHIONEIN-II GENES INCREASES SENSITIVITY TO CADMIUM [J].
MASTERS, BA ;
KELLY, EJ ;
QUAIFE, CJ ;
BRINSTER, RL ;
PALMITER, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (02) :584-588
[25]   TARGETING AND GERM-LINE TRANSMISSION OF A NULL MUTATION AT THE METALLOTHIONEIN I-LOCI AND II-LOCI IN MOUSE [J].
MICHALSKA, AE ;
CHOO, KHA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8088-8092
[26]   REGULATION OF METALLOTHIONEIN GENES BY HEAVY-METALS APPEARS TO BE MEDIATED BY A ZINC-SENSITIVE INHIBITOR THAT INTERACTS WITH A CONSTITUTIVELY ACTIVE TRANSCRIPTION FACTOR, MTF-1 [J].
PALMITER, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1219-1223
[27]   CLONED TRANSCRIPTION FACTOR MTF-1 ACTIVATES THE MOUSE METALLOTHIONEIN-I PROMOTER [J].
RADTKE, F ;
HEUCHEL, R ;
GEORGIEV, O ;
HERGERSBERG, M ;
GARIGLIO, M ;
DEMBIC, Z ;
SCHAFFNER, W .
EMBO JOURNAL, 1993, 12 (04) :1355-1362
[28]   Regulation of ZiRF1 and basal SP1 transcription factor MRE-binding activity by transition metals [J].
Remondelli, P ;
Moltedo, O ;
Leone, A .
FEBS LETTERS, 1997, 416 (03) :254-258
[29]   CADMIUM CHLORIDE (CDCL2)-INDUCED METALLOTHIONEIN (MT) EXPRESSION IN NEONATAL RAT PRIMARY ASTROCYTE CULTURES [J].
RISING, L ;
VITARELLA, D ;
KIMELBERG, HK ;
ASCHNER, M .
BRAIN RESEARCH, 1995, 678 (1-2) :91-98
[30]   Metallothionein inducers protect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicity in mice [J].
Rojas, P ;
Rios, C .
NEUROCHEMICAL RESEARCH, 1997, 22 (01) :17-22