Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein

被引：57

作者：

Noisakran, Sansanee ^{[1
,2
]}

Sengsai, Suchada ^{[1
]}

Thongboonkerd, Visith ^{[1
,3
]}

Kanlaya, Rattiyaporn ^{[1
,3
]}

Sinchaikul, Supachok ^{[4
,5
]}

Chen, Shui-Tein ^{[4
,5
,6
]}

Puttikhunt, Chunya ^{[1
,2
]}

Kasinrerk, Watchara ^{[2
,7
]}

Limjindaporn, Thawornchai ^{[1
]}

Wongwiwat, Wiyada ^{[1
]}

Malasit, Prida ^{[1
,2
]}

Yenchitsomanus, Pa-thai ^{[1
,2
]}

机构：

[1] Mahidol Univ, Fac Med Siriraj Hosp, Dept Res & Dev, Med Mol Biol Unit, Bangkok 10700, Thailand

[2] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Med Biotechnol Unit, Pathum Thani 12120, Thailand

[3] Mahidol Univ, Fac Med Siriraj Hosp, Dept Res & Dev, Med Prote Unit, Bangkok 10700, Thailand

[4] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan

[5] Acad Sinica, Genom Res Ctr, Taipei 115, Taiwan

[6] Natl Taiwan Univ, Inst Biochem Sci, Coll Life Sci, Taipei, Taiwan

[7] Chiang Mai Univ, Dept Clin Immunol, Fac Associated Med Sci, Chiang Mai 50200, Thailand

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 372卷 / 01期

关键词：

hnRNP C1/C2; dengue virus; NSI; interacting protein; host and viral protein interaction; virus replication;

D O I：

10.1016/j.bbrc.2008.04.165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：67 / 72

页数：6

共 29 条

[1]

[Anonymous], FIELDS VIROLOGY

[2] Vascular leakage in severe dengue virus infections: A potential role for the nonstructural viral protein NS1 and complement [J].