Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy

被引：26

作者：

Moers, MHP ^{[1
]}

Kalle, WHJ ^{[1
]}

Ruiter, AGT ^{[1
]}

Wiegant, JCAG ^{[1
]}

Raap, AK ^{[1
]}

Greve, J ^{[1
]}

DeGrooth, BG ^{[1
]}

VanHulst, NF ^{[1
]}

机构：

[1] LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY,2333 AL LEIDEN,NETHERLANDS

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1996年 / 182卷

关键词：

near-field scanning optical microscopy; fluorescence microscopy; fluorescence in situ hybridization; human metaphase chromosomes; DNA; sequencing;

D O I：

10.1111/j.1365-2818.1996.tb04795.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.

引用

页码：40 / 45

页数：6

共 31 条

[1]

AMBROSE SE, 1993, FOREIGN AFF, V72, P160

[2]

ANDERSON LA, 1989, CYTOGENET CELL GENET, V51, P951

[3] A NEW METHOD FOR FLUORESCENCE MICROSCOPICAL LOCALIZATION OF SPECIFIC DNA-SEQUENCES BY INSITU HYBRIDIZATION OF FLUOROCHROME-LABELED RNA [J].

BAUMAN, JGJ ;

WIEGANT, J ;

BORST, P ;

VANDUIJN, P .

EXPERIMENTAL CELL RESEARCH, 1980, 128 (02) :485-490

[4] NEAR-FIELD OPTICS - MICROSCOPY, SPECTROSCOPY, AND SURFACE MODIFICATION BEYOND THE DIFFRACTION LIMIT [J].

BETZIG, E ;

TRAUTMAN, JK .

SCIENCE, 1992, 257 (5067) :189-195

[5] COMBINED SHEAR FORCE AND NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

FINN, PL ;

WEINER, JS .

APPLIED PHYSICS LETTERS, 1992, 60 (20) :2484-2486

[6] SINGLE MOLECULES OBSERVED BY NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

CHICHESTER, RJ .

SCIENCE, 1993, 262 (5138) :1422-1425

[7]

BUONGIORNONARDE.M, 1969, NATURE, V225, P946

[8] A HYPERVARIABLE REPEATED SEQUENCE ON HUMAN CHROMOSOME-1P36 [J].

BUROKER, N ;

BESTWICK, R ;

HAIGHT, G ;

MAGENIS, RE ;

LITT, M .

HUMAN GENETICS, 1987, 77 (02) :175-181

[9] CLONING OF HUMAN SATELLITE-III DNA - DIFFERENT COMPONENTS ARE ON DIFFERENT CHROMOSOMES [J].

COOKE, HJ ;

HINDLEY, J .

NUCLEIC ACIDS RESEARCH, 1979, 6 (10) :3177-3197

[10] MULTIPLE COLORS BY FLUORESCENCE INSITU HYBRIDIZATION USING RATIO-LABELED DNA PROBES CREATE A MOLECULAR KARYOTYPE [J].

DAUWERSE, JG ;

WIEGANT, J ;

RAAP, AK ;

BREUNING, MH ;

VANOMMEN, GJB .

HUMAN MOLECULAR GENETICS, 1992, 1 (08) :593-598

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