Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography

被引：162

作者：

Dobrev, PI ^{[1
]}

Havlícek, L ^{[1
]}

Vágner, M ^{[1
]}

Malbeck, J ^{[1
]}

Kamínek, M ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Expt Bot, CR-16502 Prague, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1075卷 / 1-2期

关键词：

auxin; abscisic acid; solid phase extraction; 2D-HPLC;

D O I：

10.1016/j.chroma.2005.02.091

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：159 / 166

页数：8

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