Purification and determination of plant hormones auxin and abscisic acid using solid phase extraction and two-dimensional high performance liquid chromatography

被引：162

作者：

Dobrev, PI ^{[1
]}

Havlícek, L ^{[1
]}

Vágner, M ^{[1
]}

Malbeck, J ^{[1
]}

Kamínek, M ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Expt Bot, CR-16502 Prague, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1075卷 / 1-2期

关键词：

auxin; abscisic acid; solid phase extraction; 2D-HPLC;

D O I：

10.1016/j.chroma.2005.02.091

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for separation and purification of plant hormones auxin and abscisic acid based on mixed mode reversed-phase anion-exchange solid phase extraction and two-dimensional HPLC was developed. Two-dimensional HPLC in "heart cutting" mode was very efficient in the purification of these two hormones. Its purification power is high enough to allow reliable on-line quantification of both hormones even with non-selective detectors. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：159 / 166

页数：8

共 29 条

[21] GAS CHROMATOGRAPHY MASS SPECTROMETRIC DETERMINATIONS OF ABSCISIC-ACID LEVELS IN CAP AND APEX OF MAIZE ROOTS [J].

RIVIER, L ;

MILON, H ;

PILET, PE .

PLANTA, 1977, 134 (01) :23-27

[22]

ROTHMAN LD, 1990, MULTIDIMENSIONAL CHR, P219

[23] Simultaneous analysis of phytohormones, phytotoxins, and volatile organic compounds in plants [J].

Schmelz, EA ;

Engelberth, J ;

Alborn, HT ;

O'Donnell, P ;

Sammons, M ;

Toshima, H ;

Tumlinson, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (18) :10552-10557

[24] An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation [J].

Wagner, K ;

Miliotis, T ;

Marko-Varga, G ;

Bischoff, R ;

Unger, KK .

ANALYTICAL CHEMISTRY, 2002, 74 (04) :809-820

[25] Large-scale analysis of the yeast proteome by multidimensional protein identification technology [J].

Washburn, MP ;

Wolters, D ;

Yates, JR .

NATURE BIOTECHNOLOGY, 2001, 19 (03) :242-247

[26]

Wehr T, 2003, LC GC EUR, V16, P154

[27] Isolation of the active constituents in natural materials by 'heart-cutting' isocratic reversed-phase two-dimensional liquid chromatography [J].

Wong, V ;

Shalliker, RA .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1036 (01) :15-24

[28]

YUAN M, 1997, CHIN J CHROMATOGR, V15, P482

[29] Rapid extraction of abscisic acid and its metabolites for liquid chromatography-tandem mass spectrometry [J].

Zhou, R ;

Squires, TM ;

Ambrose, SJ ;

Abrams, SR ;

Ross, ARS ;

Cutler, AJ .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1010 (01) :75-85

← 1 2 3 →