BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2

被引：365

作者：

Lee, MH

Kwon, TG

Park, HS

Wozney, JM

Ryoo, HM ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Sch Dent, Dept Biochem, Taegu 700422, South Korea

[2] Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Taegu 700422, South Korea

[3] Kyungpook Natl Univ, Sch Dent, Dept Orthodont, Taegu 700422, South Korea

[4] Genet Inst Inc, Cambridge, MA 02140 USA

[5] Kyungpook Natl Univ, Skeletal Dis Genome Res Ctr, Taegu 700422, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 309卷 / 03期

关键词：

Osterix; BMP-2; Dlx5; Runx2; osteoblast;

D O I：

10.1016/j.bbrc.2003.08.058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BMP-2 stimulates the expression of three osteogenic master transcription factors: Runx2, D1x5, and Osterix (Osx). However, the hierarchical regulatory relationships among them are not yet clearly understood. Osx was commonly stimulated in osteogenic and non-osteogenic cells in response to BMP-signaling, as D1x5 was in our previous report. A cycloheximide experiment indicated that Osx expression by BMP-2 requires new protein synthesis. Even if Osx has been suggested as a downstream target of Runx2, the results of this study indicated that Osx expression was still induced by BMP-2 treatment in Runx2 null cells, but not induced by Runx2 overexpression in myogenic C2C12 cells. Instead, Osx expression by BMP-2 was completely abrogated by the antisense blocking of D1x5. Depending upon the coincident expression pattern of Osx and D1x5, and the blocking of Osx expression by the antisense D1x5, BMP-2-induced Osx expression is mainly mediated not by Runx2, but by D1x5. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：689 / 694

页数：6

共 27 条

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