Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion

被引：439

作者：

Atarashi, Ryuichiro ^{[1
,2
]}

Satoh, Katsuya ^{[1
]}

Sano, Kazunori ^{[1
,3
]}

Fuse, Takayuki ^{[1
]}

Yamaguchi, Naohiro ^{[1
]}

Ishibashi, Daisuke ^{[1
]}

Matsubara, Takehiro ^{[1
]}

Nakagaki, Takehiro ^{[1
]}

Yamanaka, Hitoki ^{[4
]}

Shirabe, Susumu ^{[5
]}

Yamada, Masahito ^{[6
]}

Mizusawa, Hidehiro ^{[7
]}

Kitamoto, Tetsuyuki ^{[8
]}

Klug, Genevieve ^{[9
]}

McGlade, Amelia ^{[9
]}

Collins, Steven J. ^{[9
]}

Nishida, Noriyuki ^{[1
,3
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Dept Mol Microbiol & Immunol, Nagasaki 852, Japan

[2] Nagasaki Univ, Res Ctr Genom Instabil & Carcinogenesis, Nagasaki 852, Japan

[3] Nagasaki Univ, Global Ctr Excellence Program, Nagasaki 852, Japan

[4] Nagasaki Univ, Ctr Frontier Life Sci, Div Comparat Med, Nagasaki 852, Japan

[5] Nagasaki Univ Hosp, Org Rural Med & Residency Educ, Nagasaki, Japan

[6] Kanazawa Univ, Grad Sch Med Sci, Dept Neurol & Neurobiol Aging, Kanazawa, Ishikawa, Japan

[7] Tokyo Med & Dent Univ, Grad Sch, Dept Neurol & Neurol Sci, Tokyo, Japan

[8] Tohoku Univ, Grad Sch Med, Dept Prion Res, Div Creutzfeldt Jakob Dis Sci & Technol, Sendai, Miyagi 980, Japan

[9] Univ Melbourne, Australian Natl Creutzfeldt Jakob Dis Registry, Dept Pathol, Melbourne, Vic, Australia

来源：

NATURE MEDICINE | 2011年 / 17卷 / 02期

基金：

奥地利科学基金会;

关键词：

CREUTZFELDT-JAKOB-DISEASE; CYCLIC AMPLIFICATION; SEEDED CONVERSION; 14-3-3; PROTEIN; DIAGNOSIS;

D O I：

10.1038/nm.2294

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrPSc) has generated the potential for sensitive detection of prions. Here we developed a new PrPSc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of >= 1 fg of PrPSc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.

引用

页码：175 / 178

页数：4

共 19 条

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