Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar Typhimurium

被引：37

作者：

Aldor, I ^{[1
]}

Keasling, JD ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 02期

关键词：

PHBV; PHA; polyhydroxyalkanoate; propionyl-CoA synthetase; PrpE; biodegradable plastic;

D O I：

10.1002/bit.1150

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant strain of Salmonella enterica serovar Typhimurium (mutant in propionate-activation activity) was metabolically engineered to control the composition of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a polyhydroxyalkanoate copolymer with commercially desirable properties. A gene (prpE) encoding propionyl-CoA synthetase was placed under the control of the IPTG-inducible taclacUV5 promoter (P-taclacUV5) while the polyhydroxyalkanoate synthesis operon (phaBCA) from Acinetobacter sp. RA3849 was coexpressed under the control of the arabinose-inducible araBAD promoter (P-BAD), S. enterica, harboring both constructs, was grown in medium containing a fixed substrate concentration and the composition of the copolymer was varied between 2 mol% and 25 mol% 3-hydroxyvalerate by controlling the IPTG level in the medium. This "dial-a-composition" system should find application in cases where the substrate concentration of feedstream for PHBV bioplastic production is not adjustable. (C) 2001 John Wiley & Sons, Inc.

引用

页码：108 / 114

页数：7

共 55 条

[1] Polyhydroxyalkanoates, biopolyesters from renewable resources: Physiological and engineering aspects [J].

Braunegg, G ;

Lefebvre, G ;

Genser, KF .

JOURNAL OF BIOTECHNOLOGY, 1998, 65 (2-3) :127-161

[2] POLYMER SYNTHESIS BY MICROORGANISMS - TECHNOLOGY AND ECONOMICS [J].

BYROM, D .

TRENDS IN BIOTECHNOLOGY, 1987, 5 (09) :246-250

[3]

Choi JI, 1999, APPL ENVIRON MICROB, V65, P4363

[4]

COX MK, 1994, STUD POLYM SCI, V12, P120

[5] Production of heteropolymeric polyhydroxyalkanoate in Escherichia coli from a single carbon source [J].

Eschenlauer, AC ;

Stoup, SK ;

Srienc, F ;

Somers, DA .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1996, 19 (02) :121-130

[6] POLYHYDROXYALKANOATE PRODUCTION IN RECOMBINANT ESCHERICHIA-COLI [J].

FIDLER, S ;

DENNIS, D .

FEMS MICROBIOLOGY LETTERS, 1992, 103 (2-4) :231-235

[7]

FOX DK, 1986, J BIOL CHEM, V261, P3487

[8] Characterization of 13 kDa granule-associated protein in Aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria [J].

Fukui, T ;

Kichise, T ;

Iwata, T ;

Doi, Y .

BIOMACROMOLECULES, 2001, 2 (01) :148-153

[9] OVEREXPRESSION AND PURIFICATION OF THE SOLUBLE POLYHYDROXYALKANOATE SYNTHASE FROM ALCALIGENES-EUTROPHUS - EVIDENCE FOR A REQUIRED POSTTRANSLATIONAL MODIFICATION FOR CATALYTIC ACTIVITY [J].

GERNGROSS, TU ;

SNELL, KD ;

PEOPLES, OP ;

SINSKEY, AJ ;

CSUHAI, E ;

MASAMUNE, S ;

STUBBE, J .

BIOCHEMISTRY, 1994, 33 (31) :9311-9320

[10] TIGHT REGULATION, MODULATION, AND HIGH-LEVEL EXPRESSION BY VECTORS CONTAINING THE ARABINOSE P-BAD PROMOTER [J].

GUZMAN, LM ;

BELIN, D ;

CARSON, MJ ;

BECKWITH, J .

JOURNAL OF BACTERIOLOGY, 1995, 177 (14) :4121-4130

← 1 2 3 4 5 6 →