Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar Typhimurium

被引：37

作者：

Aldor, I ^{[1
]}

Keasling, JD ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 02期

关键词：

PHBV; PHA; polyhydroxyalkanoate; propionyl-CoA synthetase; PrpE; biodegradable plastic;

D O I：

10.1002/bit.1150

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant strain of Salmonella enterica serovar Typhimurium (mutant in propionate-activation activity) was metabolically engineered to control the composition of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a polyhydroxyalkanoate copolymer with commercially desirable properties. A gene (prpE) encoding propionyl-CoA synthetase was placed under the control of the IPTG-inducible taclacUV5 promoter (P-taclacUV5) while the polyhydroxyalkanoate synthesis operon (phaBCA) from Acinetobacter sp. RA3849 was coexpressed under the control of the arabinose-inducible araBAD promoter (P-BAD), S. enterica, harboring both constructs, was grown in medium containing a fixed substrate concentration and the composition of the copolymer was varied between 2 mol% and 25 mol% 3-hydroxyvalerate by controlling the IPTG level in the medium. This "dial-a-composition" system should find application in cases where the substrate concentration of feedstream for PHBV bioplastic production is not adjustable. (C) 2001 John Wiley & Sons, Inc.

引用

页码：108 / 114

页数：7

共 55 条

[31] pryR, ntrA, and ihf functions are required for expression of the prpBCDE operon, encoding enzymes that catabolize propionate in Salmonella enterica serovar Typhimurium LT2 [J].

Palacios, S ;

Escalante-Semerena, JC .

JOURNAL OF BACTERIOLOGY, 2000, 182 (04) :905-910

[32] PRODUCTION OF POLYHYDROXYALKANOATES, A FAMILY OF BIODEGRADABLE PLASTICS AND ELASTOMERS, IN BACTERIA AND PLANTS [J].

POIRIER, Y ;

NAWRATH, C ;

SOMERVILLE, C .

BIO-TECHNOLOGY, 1995, 13 (02) :142-150

[33] THE FUNCTION OF ACKA AND PTA GENES IS NECESSARY FOR POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) SYNTHESIS IN RECOMBINANT PHA(+) ESCHERICHIA-COLI [J].

RHIE, HG ;

DENNIS, D .

CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 :200-206

[34] ROLE OF FADR AND ATOC(CON) MUTATIONS IN POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) SYNTHESIS IN RECOMBINANT PHA(+) ESCHERICHIA-COLI [J].

RHIE, HG ;

DENNIS, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2487-2492

[35] GAS-CHROMATOGRAPHIC DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS AFTER HYDROCHLORIC-ACID PROPANOLYSIS [J].

RIIS, V ;

MAI, W .

JOURNAL OF CHROMATOGRAPHY, 1988, 445 (01) :285-289

[36]

Sambrook J., 1989, MOL CLONING

[37] PHOSPHATE CONCENTRATION REGULATES TRANSCRIPTION OF THE ACINETOBACTER POLYHYDROXYALKANOIC ACID BIOSYNTHETIC GENES [J].

SCHEMBRI, MA ;

BAYLY, RC ;

DAVIES, JK .

JOURNAL OF BACTERIOLOGY, 1995, 177 (15) :4501-4507

[38] IDENTIFICATION OF A 13-KDA PROTEIN ASSOCIATED WITH THE POLYHYDROXYALKANOIC ACID GRANULES FROM ACINETOBACTER SPP [J].

SCHEMBRI, MA ;

WOODS, AA ;

BAYLY, RC ;

DAVIES, JK .

FEMS MICROBIOLOGY LETTERS, 1995, 133 (03) :277-283

[39] Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations [J].

Siegele, DA ;

Hu, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (15) :8168-8172

[40] PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo [J].

Sim, SJ ;

Snell, KD ;

Hogan, SA ;

Stubbe, J ;

Rha, CK ;

Sinskey, AJ .

NATURE BIOTECHNOLOGY, 1997, 15 (01) :63-67

← 1 2 3 4 5 6 →