Enzymatic synthesis of [1-13C]- and [1-14C]-L-phenylalanine

被引：14

作者：

Jemielity, J ^{[1
]}

Kanska, M ^{[1
]}

Kanski, R ^{[1
]}

机构：

[1] Warsaw Univ, Dept Chem, PL-02093 Warsaw, Poland

来源：

ISOTOPES IN ENVIRONMENTAL AND HEALTH STUDIES | 1998年 / 34卷 / 04期

关键词：

amino acids; carbon-13; carbon-14; enzyme reaction; stereospecificity;

D O I：

10.1080/10256019808234070

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The two isotopomers of L-phenylalanine labelled with C-13 or C-14 in the carboxylic group have been obtained by the combined chemical and enzymatic methods. [1-C-13]- or [1-C-14]-L-phenylalanine have been synthesised by conversion of [1-C-13]- or [1-C-14]CH3COONa into proper cinnamic acid species followed by ammonia addition in the presence of phenylalanine ammonia lyase, The reaction yield has been determined enzymatically and spectroscopically; radiochemical yield of C-14-compound has been determined by liquid scintillation counting, and the C-13 enrichment level has been established by C-13 NMR and mass spectroscopy methods.

引用

页码：335 / 339

页数：5