Escherichia coli O157:H7 in retail ground beef in Seattle:: Results of a one-year prospective study

被引：41

作者：

Tarr, PI ^{[1
]}

Tran, NT

Wilson, RA

机构：

[1] Childrens Hosp & Med Ctr, Div Gastroenterol, Seattle, WA 98105 USA

[2] Univ Washington, Sch Med, Dept Pediat, Seattle, WA 98105 USA

[3] Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98105 USA

来源：

JOURNAL OF FOOD PROTECTION | 1999年 / 62卷 / 02期

关键词：

D O I：

10.4315/0362-028X-62.2.133

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli O157:H7 was sought systematically in 1,400 samples of retail ground beef in Seattle in a 1-year prospective study. Sorbitol-nonfermenting, lactose-fermenting, indole-positive colonies isolated after enrichment culture were probed for the presence of Shiga toxin genes. Totals of 67,040 sorbitol-nonfermenting and 66,705 sorbitol-fermenting colonies were characterized, but E. coli O157:H7 was not identified. The sensitivity of this technique was usually sufficient to detect E. coli O157:H7 at a concentration below 1 CFU/g of meat. These data demonstrate that retail ground beef in Seattle is neither frequently nor heavily contaminated with E. coli O157:H7.

引用

页码：133 / 139

页数：7

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