Identification of single tiny seeds of Orobanche using RAPD analysis

被引：7

作者：

Joel, DM ^{[1
]}

Portnoy, V ^{[1
]}

Katzir, N ^{[1
]}

机构：

[1] AGR RES ORG,NEWE YAAR RES CTR,IL-30095 RAMAT YISHAY,ISRAEL

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1996年 / 14卷 / 03期

关键词：

DNA extraction; RAPD-PCR; seed; Orobanche; parasitic plant;

D O I：

10.1007/BF02671659

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The tiny seeds of parasitic weeds of the genus Orobanche can be identified by using RAPD markers. A simple procedure for DNA extraction from single seeds, 10 mu g each, followed by RAPD-PCR and using specific DNA markers, leads to species identification. Seeds of five different species could be identified using this method.

引用

页码：243 / 248

页数：6

共 7 条

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[3]

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[4]

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[5]

KATZIR N, 1996, IN PRESS THEOR APPL

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Parker C., 1993, Parasitic weeds of the world: biology and control

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