Surface plasmon resonance imaging-based protein array chip system for monitoring a hexahistidine-tagged protein during expression and purification

被引：8

作者：

Ro, HS ^{[1
]}

Jung, SO ^{[1
]}

Kho, BH ^{[1
]}

Hong, HP ^{[1
]}

Lee, JS ^{[1
]}

Shin, YB ^{[1
]}

Kim, MG ^{[1
]}

Chung, BH ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, BioNanotechnol Res Ctr, Taejon 305600, South Korea

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 02期

关键词：

D O I：

10.1128/AEM.71.2.1089-1092.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A surface plasmon resonance imaging-based Ni2+-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

引用

页码：1089 / 1092

页数：4

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