Application of CCC for the separation of lutein from a crude extract of marigold flower petals

被引：18

作者：

Wei, Y ^{[1
]}

Zhang, TY

Xu, GQ

Ito, Y

机构：

[1] Beijing Inst New Technol Applicat, Beijing Res Ctr Separat & Purificat Technol Nat P, Beijing 100035, Peoples R China

[2] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 9-10期

关键词：

lutein; countercurrent chromatography; carotenoids; marigold flower petal;

D O I：

10.1081/JLC-120021274

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Analytical high-speed countercurrent chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate lutein from the crude extract of the marigold flower petal. Lutein was obtained by preparative HSCCC with a two-phase solvent system composed of n-heptane-chloroform-acetonitrile (10 : 3: 7, v/v/v). HPLC analyses of the lutein revealed that it was over 98.5% purity. The final identification was performed by UV-VIS absorption, H-1-NMR, and C-13-NMR Spectra.

引用

页码：1659 / 1669

页数：11

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