Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography

被引：65

作者：

Li, HB

Chen, F

Zhang, TY

Yang, FQ

Xu, GQ

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

[2] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 905卷 / 1-2期

关键词：

counter-current chromatography; Chlorella vulgaris; preparative chromatography; lutein; carotenoids; vitamins;

D O I：

10.1016/S0021-9673(00)00987-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 155

页数：5

共 12 条

[1] Separation and purification of isoflavones from Pueraria lobata by high-speed counter-current chromatography [J].