Dimeric PKD regulates membrane fission to form transport carriers at the TGN

被引：116

作者：

Bossard, Carine ^{[1
]}

Bresson, Damien ^{[2
]}

Polishchuk, Roman S. ^{[3
]}

Malhotra, Vivek ^{[1
]}

机构：

[1] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

[2] La Jolla Inst Allergy & Immunol, La Jolla, CA 92037 USA

[3] Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Santa Maria Imbaro, Italy

来源：

JOURNAL OF CELL BIOLOGY | 2007年 / 179卷 / 06期

关键词：

D O I：

10.1083/jcb.200703166

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Protein kinase D (PKD) is recruited to the trans-Golgi network (TGN) through interaction with diacylglycerol (DAG) and is required for the biogenesis of TGN to cell surface transport carriers. We now provide definitive evidence that PKD has a function in membrane fission. PKD depletion by siRNA inhibits trafficking from the TGN, whereas expression of a constitutively active PKD converts TGN into small vesicles. These findings demonstrate that PKD regulates membrane fission and this activity is used to control the size of transport carriers, and to prevent uncontrolled vesiculation of TGN during protein transport.

引用

页码：1123 / 1131

页数：9

共 25 条

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