Multiphoton plasmon-resonance microscopy

被引：210

作者：

Yelin, D ^{[1
]}

Oron, D ^{[1
]}

Thiberge, S ^{[1
]}

Moses, E ^{[1
]}

Silberberg, Y ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Phys Complex Syst, IL-76100 Rehovot, Israel

来源：

OPTICS EXPRESS | 2003年 / 11卷 / 12期

关键词：

D O I：

10.1364/OE.11.001385

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A novel method for detection of noble-metal nanoparticles by their nonlinear optical properties is presented and applied for specific labeling of cellular organelles. When illuminated by laser light in resonance with their plasmon frequency these nanoparticles generate an enhanced multiphoton signal. This enhanced signal is measured to obtain a depth-resolved image in a laser scanning microscope setup. Plasmon-resonance images of both live and fixed cells, showing specific labeling of cellular organelles and membranes, either by two-photon autofluorescence or by third-harmonic generation, are presented. (C) 2003 Optical Society of America.

引用

页码：1385 / 1391

页数：7

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