Selective inhibition of the growth of incompatible pollen tubes by S-protein in the Japanese pear

被引:87
作者
Hiratsuka, S [1 ]
Zhang, SL
Nakagawa, E
Kawai, Y
机构
[1] Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan
[2] Nanjing Agr Univ, Dept Hort, Nanjing 210095, Peoples R China
来源
SEXUAL PLANT REPRODUCTION | 2001年 / 13卷 / 04期
关键词
Japanese pear; pollen inhibition; Pyrus serotina; RNase; S-protein;
D O I
10.1007/s004970000058
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The S-allele-associated proteins (S-proteins) in the styles of the Japanese pear (Pyrus serotina Rehd. var. culta Rehd.) were purified by cation exchange chromatography. Their inhibitory action on the growth of incompatible pollen tubes (pollen tubes bearing the same S-allele as in the style from which the S-proteins were prepared) was characterized in vitro. Germination and tube growth of self-pollen (pollen from the same cultivar from which the S-proteins were prepared) decreased dose-dependently when the S-protein was added to the medium. Tube length was reduced to 10% that of compatible pollen tubes (pollen tubes bearing the S-allele different from that in the style from which the S-proteins were prepared) at 1.5 mug mul(1). S-proteins from Shinsui (S4S5) also inhibited growth of cross-incompatible Kosui (S4S5) pollen tubes, but not of compatible Chojuro (S2S3) pollen tubes. After inactivation of RNase of the S-protein, the inhibitory action of the S-protein disappeared. These results indicate that the S-protein acts directly to inhibit growth of incompatible pollen tubes in Japanese pear styles, and that the RNase activity of the protein is essential for the biological function. However, small amounts of proteins that co-migrated with the S-protein may also play some roles in the inhibition. This is the first report on the selective inhibitory action of S-proteins in Rosaceae.
引用
收藏
页码:209 / 215
页数:7
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