Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

被引：62

作者：

Fitzgerald, AM

Mudge, AM

Gleave, AP

Plummer, KM

机构：

[1] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand

[2] New Zealand Ltd, Gene Technol, Hort & Food Res Inst, Auckland, New Zealand

来源：

MYCOLOGICAL RESEARCH | 2003年 / 107卷

关键词：

D O I：

10.1017/S0953756203008086

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the development of two new transformation systems, Polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.

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页码：803 / 810

页数：8

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