Efficient primer strand extension beyond oxadiazole carboxamide nucleobases

被引：19

作者：

Adelfinskaya, O

Nashine, VC

Bergstrom, DE

Davisson, VJ ^{[1
]}

机构：

[1] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA

[2] Walther Canc Inst, Indianapolis, IN 46208 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 46期

关键词：

D O I：

10.1021/ja054226j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two oxadiazole carboxamide deoxyribonucleoside analogues are described that can be incorporated and efficiently extended by Taq DNA polymerase. The primer strand extension beyond oxadiazole nucleoside analogues occurs at rates similar to the values observed for the canonical Watson-Crick base pairs irrespective of the template nucleobase. These distinctive chemical effects in DNA polymerase extensions are attributed to the smaller size and unique electronic properties of the oxadiazole nucleobase. Copyright © 2005 American Chemical Society.

引用

页码：16000 / 16001

页数：2