Rapid monitoring of arrays of amino acids in clinical samples using capillary electrophoresis with contactless conductivity detection

被引：71

作者：

Tuma, Petr ^{[1
]}

Malkova, Klara ^{[1
,2
]}

Samcova, Eva ^{[1
]}

Stulik, Karel ^{[2
]}

机构：

[1] Charles Univ Prague, Inst Biochem Cell & Mol Biol, Fac Med 3, Prague 10000 10, Czech Republic

[2] Charles Univ Prague, Dept Analyt Chem, CR-11636 Prague 1, Czech Republic

来源：

JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 16期

关键词：

Amino acids; Clinical samples; Contactless conductivity detection; Short pathway separation; LASER-INDUCED FLUORESCENCE; ZONE-ELECTROPHORESIS; HUMAN PLASMA; SEPARATION; CE; OPTIMIZATION; DIAGNOSIS;

D O I：

10.1002/jssc.201000137

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

CE with contactless conductivity detection has been used to separate 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the tested amino acids can be mutually separated in 0.5-10 mol/L acetic acid electrolytes. The time of analysis does not exceed 6 min and the LODs vary from 0.1 to 1.7 mu mol/L. The CVs lie within the intervals 0.01-0.4% and 0.9-4% for the migration times and the analyte peak areas, respectively. The procedure has been successfully applied to the determinations of the whole amino acid spectra in blood plasma, urine, saliva and cerebrospinal fluid samples.

引用

页码：2394 / 2401

页数：8

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