Determination of choline-containing phospholipids in serum with a fiber-optic biosensor

被引：25

作者：

Marazuela, MD ^{[1
]}

Moreno-Bondi, MC ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, E-28040 Madrid, Spain

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 374卷 / 01期

关键词：

biosensor; choline; phosphatidylcholine; optical fiber; oxygen optode;

D O I：

10.1016/S0003-2670(98)00413-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An optical fiber biosensor for choline-containing phospholipids monitoring in serum samples is described. Phospholipids are hydrolyzed by the enzyme phospholipase-D to choline which is analyzed with a fiber-optic biosensor based on choline oxidase and oxygen transduction. Several supports have been evaluated for enzyme immobilization. The optimum biosensing layer consists of the enzyme immobilized on a nylon membrane and placed onto an oxygen sensitive layer formed by a luminescent Ru(II) complex, tris(4,-diphenyl-1,10-phenanthroline)ruthenium(II), dispersed in a silicone membrane. The performance of the biosensor has been optimized using a flow injection system. The linear dynamic range of the biosensing membranes is found to be 0.08-3.00 mg ml(-1) phosphatidylcholine. Studies of the reproducibility, stability and interferences of the device, as well as the application of the sensor to measurements in serum samples, are reported. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：19 / 29

页数：11

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