Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent

被引：103

作者：

Molina, Sonia ^{[1
,2
,10
]}

Castet, Valerie ^{[1
,2
]}

Pichard-Garcia, Lydiane ^{[1
,2
]}

Wychowski, Czeslaw ^{[1
,3
,4
]}

Meurs, Eliane ^{[1
,5
]}

Pascussi, Jean-Marc ^{[1
,2
]}

Sureau, Camille ^{[1
,6
]}

Fabre, Jean-Michel ^{[1
,2
,7
]}

SaCunha, Antonio ^{[1
,8
]}

Larrey, Dominique ^{[1
,2
,9
]}

Dubuisson, Jean ^{[1
,3
,4
]}

Coste, Joliette ^{[1
,2
,10
]}

McKeating, Jane ^{[1
,11
]}

Maurel, Patrick ^{[1
,2
]}

Fournier-Wirth, Chantal ^{[1
,2
,10
]}

机构：

[1] INSERM, U632, F-34293 Montpellier 5, France

[2] Univ Montpellier 1, EA3768, F-34000 Montpellier, France

[3] Inst Biol, Unit Hepatite C, UPR2511, CNRS, F-59021 Lille, France

[4] Inst Pasteur, F-59021 Lille, France

[5] Inst Pasteur, Unit Hepacivirus, F-75724 Paris, France

[6] Inst Natl Transfus Sanguine, F-75139 Paris, France

[7] Hop St Eloi, Serv Chirurgie Digest, F-34095 Montpellier, France

[8] Hop Haut Leveque, Serv Chirurgie Digest, F-33075 Pessac, France

[9] Hop St Eloi, Serv Hepatogastroenterol, F-34095 Montpellier, France

[10] Etablissement Francais Sang, F-34094 Montpellier, France

[11] Univ Birmingham, Inst Biomed Res, Div Immunol & Infect, Birmingham B15 2TT, W Midlands, England

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1128/JVI.01443-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

引用

页码：569 / 574

页数：6

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