Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent

被引：103

作者：

Molina, Sonia ^{[1
,2
,10
]}

Castet, Valerie ^{[1
,2
]}

Pichard-Garcia, Lydiane ^{[1
,2
]}

Wychowski, Czeslaw ^{[1
,3
,4
]}

Meurs, Eliane ^{[1
,5
]}

Pascussi, Jean-Marc ^{[1
,2
]}

Sureau, Camille ^{[1
,6
]}

Fabre, Jean-Michel ^{[1
,2
,7
]}

SaCunha, Antonio ^{[1
,8
]}

Larrey, Dominique ^{[1
,2
,9
]}

Dubuisson, Jean ^{[1
,3
,4
]}

Coste, Joliette ^{[1
,2
,10
]}

McKeating, Jane ^{[1
,11
]}

Maurel, Patrick ^{[1
,2
]}

Fournier-Wirth, Chantal ^{[1
,2
,10
]}

机构：

[1] INSERM, U632, F-34293 Montpellier 5, France

[2] Univ Montpellier 1, EA3768, F-34000 Montpellier, France

[3] Inst Biol, Unit Hepatite C, UPR2511, CNRS, F-59021 Lille, France

[4] Inst Pasteur, F-59021 Lille, France

[5] Inst Pasteur, Unit Hepacivirus, F-75724 Paris, France

[6] Inst Natl Transfus Sanguine, F-75139 Paris, France

[7] Hop St Eloi, Serv Chirurgie Digest, F-34095 Montpellier, France

[8] Hop Haut Leveque, Serv Chirurgie Digest, F-33075 Pessac, France

[9] Hop St Eloi, Serv Hepatogastroenterol, F-34095 Montpellier, France

[10] Etablissement Francais Sang, F-34094 Montpellier, France

[11] Univ Birmingham, Inst Biomed Res, Div Immunol & Infect, Birmingham B15 2TT, W Midlands, England

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1128/JVI.01443-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

引用

页码：569 / 574

页数：6

共 46 条

[21] DEMONSTRATION OF IN-VITRO INFECTION OF CHIMPANZEE HEPATOCYTES WITH HEPATITIS-C VIRUS USING STRAND-SPECIFIC RT/PCR [J].

LANFORD, RE ;

SUREAU, C ;

JACOB, JR ;

WHITE, R ;

FUERST, TR .

VIROLOGY, 1994, 202 (02) :606-614

[22] Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus [J].

Lavillette, D ;

Tarr, AW ;

Voisset, C ;

Donot, P ;

Bartosch, B ;

Bain, C ;

Patel, AH ;

Dubuisson, J ;

Ball, JK ;

Cosset, FL .

HEPATOLOGY, 2005, 41 (02) :265-274

[23] Complete replication of hepatitis C virus in cell culture [J].

Lindenbach, BD ;

Evans, MJ ;

Syder, AJ ;

Wölk, B ;

Tellinghuisen, TL ;

Liu, CC ;

Maruyama, T ;

Hynes, RO ;

Burton, DR ;

McKeating, JA ;

Rice, CM .

SCIENCE, 2005, 309 (5734) :623-626

[24] DC-SIGN and L-SIGN are high affinity binding receptors for hepatitis C virus glycoprotein E2 [J].

Lozach, PY ;

Lortat-Jacob, H ;

de Lavalette, AD ;

Staropoli, I ;

Foung, S ;

Amara, A ;

Houlès, C ;

Fieschi, F ;

Schwartz, O ;

Virelizier, JL ;

Arenzana-Seisdedos, F ;

Altmeyer, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (22) :20358-20366

[25] EXTRAORDINARILY LOW-DENSITY OF HEPATITIS-C VIRUS ESTIMATED BY SUCROSE DENSITY GRADIENT CENTRIFUGATION AND THE POLYMERASE CHAIN-REACTION [J].

MIYAMOTO, H ;

OKAMOTO, H ;

SATO, K ;

TANAKA, T ;

MISHIRO, S .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :715-718

[26] The low-density lipoprotein receptor plays a role in the infection of primary human hepatocytes by hepatitis C virus [J].

Molina, Sonia ;

Castet, Valerie ;

Fournier-Wirth, Chantal ;

Pichard-Garcia, Lydiane ;

Avner, Rachel ;

Harats, Dror ;

Roitelman, Joseph ;

Barbaras, Ronald ;

Graber, Pierre ;

Ghersa, Paola ;

Smolarsky, Moshe ;

Funaro, Ada ;

Malavasi, Fabio ;

Larrey, Dominique ;

Coste, Joliette ;

Fabre, Jean-Michel ;

Sa-Cunha, Antonio ;

Maurel, Patrick .

JOURNAL OF HEPATOLOGY, 2007, 46 (03) :411-419

[27] Identification of host genes involved in hepatitis C virus replication by small interfering RNA technology [J].

Ng, Teresa I. ;

Mo, Hongmei ;

Pilot-Matias, Tami ;

He, Yupeng ;

Koev, Gennadiy ;

Krishnan, Preethi ;

Mondal, Rubina ;

Pithawalla, Ron ;

He, Wenping ;

Dekhtyar, Tanya ;

Packer, Jeremy ;

Schurdak, Mark ;

Molla, Akhteruzzaman .

HEPATOLOGY, 2007, 45 (06) :1413-1421

[28] Possible involvement of pregnane X receptor-enhanced CYP24 expression in drug-induced osteomalacia [J].

Pascussi, JM ;

Robert, A ;

Nguyen, M ;

Wairant-Debray, O ;

Garabedian, M ;

Martin, P ;

Pineau, T ;

Saric, J ;

Navarro, F ;

Maurel, P ;

Vilarem, MJ .

JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (01) :177-186

[29] Structure-function analysis of hepatitis C virus envelope-CD81 binding [J].

Petracca, R ;

Falugi, F ;

Galli, G ;

Norais, N ;

Rosa, D ;

Campagnoli, S ;

Burgio, V ;

Di Stasio, E ;

Giardina, B ;

Houghton, M ;

Abrignani, S ;

Grandi, G .

JOURNAL OF VIROLOGY, 2000, 74 (10) :4824-4830

[30]

Pichard Lydiane, 2006, Methods Mol Biol, V320, P283

← 1 2 3 4 5 →