A deep profiler's guide to cytometry

被引：510

作者：

Bendall, Sean C. ^{[2
]}

Nolan, Garry P. ^{[2
]}

Roederer, Mario ^{[1
]}

Chattopadhyay, Pratip K. ^{[1
]}

机构：

[1] NIAID, ImmunoTechnol Sect, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

[2] Stanford Univ, Baxter Lab Stem Cell Biol, Dept Microbiol & Immunol, Stanford, CA 94305 USA

来源：

TRENDS IN IMMUNOLOGY | 2012年 / 33卷 / 07期

关键词：

fluorescence; inductively coupled plasma mass spectrometry; single cell analysis; immunophenotyping; data analysis; ELEMENT-TAGGED IMMUNOASSAY; FLOW-CYTOMETRY; MASS CYTOMETRY; CYTOKINE EXPRESSION; MULTIPLEX BIOASSAY; T-CELLS; POLYMER; IDENTIFICATION; PHYCOERYTHRIN; LYMPHOCYTES;

D O I：

10.1016/j.it.2012.02.010

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes in individual cells. The ability to measure simultaneously multiple genes in the same cell is necessary to resolve the great diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can quantify 18 proteins per cell, at >10 000 cells/s. Mass cytometry is a new technology that promises to extend these capabilities significantly. Immunophenotyping by mass spectrometry provides the ability to measure >36 proteins at a rate of 1000 cells/s. We review these cytometric technologies, capable of high-content, high-throughput single-cell assays.

引用

页码：323 / 332

页数：10

共 68 条

[1] Metal-containing polystyrene beads as standards for mass cytometry [J].

Abdelrahman, Ahmed I. ;

Ornatsky, Olga ;

Bandura, Dmitry ;

Baranov, Vladimir ;

Kinach, Robert ;

Dai, Sheng ;

Thickett, Stuart C. ;

Tanner, Scott ;

Winnik, Mitchell A. .

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2010, 25 (03) :260-268

[2] Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays* [J].

Aghaeepour, Nima ;

Chattopadhyay, Pratip K. ;

Ganesan, Anuradha ;

O'Neill, Kieran ;

Zare, Habil ;

Jalali, Adrin ;

Hoos, Holger H. ;

Roederer, Mario ;

Brinkman, Ryan R. .

BIOINFORMATICS, 2012, 28 (07) :1009-1016

[3]

Bagwell CB, 2011, METHODS MOL BIOL, V699, P31, DOI 10.1007/978-1-61737-950-5_2

[4] Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry [J].

Bandura, Dmitry R. ;

Baranov, Vladimir I. ;

Ornatsky, Olga I. ;

Antonov, Alexei ;

Kinach, Robert ;

Lou, Xudong ;

Pavlov, Serguei ;

Vorobiev, Sergey ;

Dick, John E. ;

Tanner, Scott D. .

ANALYTICAL CHEMISTRY, 2009, 81 (16) :6813-6822

[5] A sensitive and quantitative element-tagged immunoassay with ICPMS detection [J].

Baranov, VI ;

Quinn, Z ;

Bandura, DR ;

Tanner, SD .

ANALYTICAL CHEMISTRY, 2002, 74 (07) :1629-1636

[6] Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum [J].

Bendall, Sean C. ;

Simonds, Erin F. ;

Qiu, Peng ;

Amir, El-ad D. ;

Krutzik, Peter O. ;

Finck, Rachel ;

Bruggner, Robert V. ;

Melamed, Rachel ;

Trejo, Angelica ;

Ornatsky, Olga I. ;

Balderas, Robert S. ;

Plevritis, Sylvia K. ;

Sachs, Karen ;

Pe'er, Dana ;

Tanner, Scott D. ;

Nolan, Garry P. .

SCIENCE, 2011, 332 (6030) :687-696

[7] Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: Fluorescence of the dyes and their bioconjugates [J].

Berlier, JE ;

Rothe, A ;

Buller, G ;

Bradford, J ;

Gray, DR ;

Filanoski, BJ ;

Telford, WG ;

Yue, S ;

Liu, JX ;

Cheung, CY ;

Chang, W ;

Hirsch, JD ;

Beechem, JM ;

Haugland, RP ;

Haugland, RP .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2003, 51 (12) :1699-1712

[8] Expression of ectonucleotidase CD39 by Foxp3+ Treg cells:: hydrolysis of extracellular ATP and immune suppression [J].

Borsellino, Giovanna ;

Kleinewietfeld, Markus ;

Di Mitri, Diletta ;

Sternjak, Alexander ;

Diamantini, Adamo ;

Giometto, Raffaella ;

Hoepner, Sabine ;

Centonze, Diego ;

Bernardi, Giorgio ;

Dell'Acqua, Maria Luisa ;

Rossini, Paolo Maria ;

Battistini, Luca ;

Rotzschke, Olaf ;

Falk, Kirsten .

BLOOD, 2007, 110 (04) :1225-1232

[9]

Centers for Disease Control (CDC), 1982, MMWR Morb Mortal Wkly Rep, V31, P507

[10] Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments [J].

Chattopadhyay, Pratip K. ;

Gaylord, Brent ;

Palmer, Adrian ;

Jiang, Nan ;

Raven, Mary A. ;

Lewis, Geoff ;

Reuter, Morgan A. ;

Rahman, A. K. M. Nur-ur ;

Price, David A. ;

Betts, Michael R. ;

Roederer, Mario .

CYTOMETRY PART A, 2012, 81A (06) :456-466

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