DNA fingerprinting of strawberry (Fragaria x ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers

被引：41

作者：

Degani, C

Rowland, LJ ^{[1
]}

Levi, A

Hortynski, JA

Galletta, GJ

机构：

[1] ARS, Fruit Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA

[2] Agr Res Org, Volcani Ctr, Inst Hort, IL-50250 Bet Dagan, Israel

[3] Univ Agr, Dept Genet & Hort Plant Breeding, PL-20950 Lublin, Poland

来源：

EUPHYTICA | 1998年 / 102卷 / 02期

关键词：

DNA fingerprinting; Flagaria x ananassa; genetic diversity; genetic similarity; RAPD markers; strawberry;

D O I：

10.1023/A:1018385715007

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Forty-one of the major strawberry (Fragaria x ananassa Duch.) cultivars grown in the United States and Canada were examined for RAPD (randomly amplified polymorphic DNA) marker polymorphisms using 10mer primers (>50% GC content). A set of 10 primers produced 15 polymorphic fragments ranging in size between 450 and 1200 bp, which were more than sufficient to distinguish among all tested cultivars. Ten of the markers derived from seven primers were absolutely required for distinguishing the cultivars. A DNA fingerprinting table was constructed based on these results. In addition, similarity coefficients were calculated based on RAPD marker data and a dendogram was constructed using the unweighted pair group method of arithmetic averages (UPGMA). These results were compared with known pedigree data for the cultivars. Our results demonstrate that RAPD markers can be used effectively for strawberry cultivar identification.

引用

页码：247 / 253

页数：7

共 23 条

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Levi A, 1994, ADV STRAWBERRY RES, V13, P36

[12] ISOZYMES AS MARKERS FOR IDENTIFICATION OF TISSUE-CULTURE AND GREENHOUSE-GROWN STRAWBERRY CULTIVARS [J].