Nucleic acid immobilized polypyrrole-polyvinylsulphonate film for Mycobacterium tuberculosis detection

被引：31

作者：

Prabhakar, Nirmal ^{[1
,2
]}

Singh, Harpal ^{[2
]}

Malhotra, Bansi D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Indian Inst Technol, Ctr Biomed Engn, New Delhi 110016, India

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 06期

关键词：

PNA biosensor; hybridization; Mycobacterium tuberculosis; polypyrrole-polyvinylsulfonate;

D O I：

10.1016/j.elecom.2008.03.006

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

21-mer peptide acid nucleic acid (PNA) probe specific to 16s-23s rRNA spacer region of Mycobacterium tuberculosis has been covalently immobilized on polypyrrole-polyvinylsulphonate film electro-chemically deposited onto indium-tin-oxide (ITO) glass for detection of complementary target by monitoring guanine oxidation and redox indicators (methylene blue and ruthenium complex) up to 0.1 fmole, 0.1 attomole and 1.0 pmole, respectively within 30 s of hybridization time. The peptide nucleic acid immobilized polypyrrole-polyvinylsulphonate electrode can be used for hybridization detection with complementary sequence in heat-shocked genomic DNA and in serum samples containing genomic M. tuberculosis DNA up to 2.5 pg/mu l within about 60 min at 30 degrees C and can be used 8-9 times. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：821 / 826

页数：6

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