Fabrication of oligonucleotide chips by using parallel cantilever-based electrochemical deposition in picoliter volumes

被引:34
作者
Descamps, Emeline
Leichle, Thierry
Corso, Bruno
Laurent, Sebastien
Mailley, Pascal
Nicu, Liviu
Livache, Thierry
Bergaud, Christian
机构
[1] CEA Grenoble, DRFMC, CNRS, UMR 5819,SPrAM, F-38054 Grenoble 9, France
[2] CNRS, LAAS, Nanobiotechnol Grp, F-31077 Toulouse, France
关键词
D O I
10.1002/adma.200602152
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The use of a parallel cantilever-based deposition system as an electrospotting tool in picoliter volumes is demonstrated. Applied in conjunction with polypyrrole electrochemistry, this generic approach for fabricating biochips is optimized for oligonucleotide chips featuring micrometer spots of different sequences with high reproducibility and specificity (see figure).
引用
收藏
页码:1816 / +
页数:7
相关论文
共 28 条
  • [1] Fabrication of biological microarrays using microcantilevers
    Belaubre, P
    Guirardel, M
    Garcia, G
    Pourciel, JB
    Leberre, V
    Dagkessamanskaia, A
    Trévisiol, E
    François, JM
    Bergaud, C
    [J]. APPLIED PHYSICS LETTERS, 2003, 82 (18) : 3122 - 3124
  • [2] Bernard A, 2000, ADV MATER, V12, P1067, DOI 10.1002/1521-4095(200007)12:14<1067::AID-ADMA1067>3.0.CO
  • [3] 2-M
  • [4] Clinically related protein-peptide interactions monitored in real time on novel peptide chips by surface plasmon resonance imaging
    Cherif, B
    Roget, A
    Villiers, CL
    Calemczuk, R
    Leroy, V
    Marche, PN
    Livache, T
    Villiers, MB
    [J]. CLINICAL CHEMISTRY, 2006, 52 (02) : 255 - 262
  • [5] Biosensors based on electropolymerized films: new trends
    Cosnier, S
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 377 (03) : 507 - 520
  • [6] Biotinylated polypyrrole films: an easy electrochemical approach for the reagentless immobilization of bacteria on electrode surfaces
    Da Silva, S
    Grosjean, L
    Ternan, N
    Mailley, P
    Livache, T
    Cosnier, S
    [J]. BIOELECTROCHEMISTRY, 2004, 63 (1-2) : 297 - 301
  • [7] Microfluidics for processing surfaces and miniaturizing biological assays
    Delamarche, E
    Juncker, D
    Schmid, H
    [J]. ADVANCED MATERIALS, 2005, 17 (24) : 2911 - 2933
  • [8] Patterning: Principles and some new developments
    Geissler, M
    Xia, YN
    [J]. ADVANCED MATERIALS, 2004, 16 (15) : 1249 - 1269
  • [9] Application of conducting polymers to biosensors
    Gerard, M
    Chaubey, A
    Malhotra, BD
    [J]. BIOSENSORS & BIOELECTRONICS, 2002, 17 (05) : 345 - 359
  • [10] A polypyrrole protein microarray for antibody-antigen interaction studies using a label-free detection process
    Grosjean, L
    Cherif, B
    Mercey, E
    Roget, A
    Levy, Y
    Marche, PN
    Villiers, MB
    Livache, T
    [J]. ANALYTICAL BIOCHEMISTRY, 2005, 347 (02) : 193 - 200