Carbon nanotubes embedded in embryoid bodies direct cardiac differentiation

被引：33

作者：

Ahadian, Samad ^{[1
]}

Yamada, Shukuyo ^{[2
]}

Estili, Mehdi ^{[3
]}

Liang, Xiaobin ^{[1
]}

Sadeghian, Ramin Banan ^{[1
,4
]}

Nakajima, Ken ^{[1
]}

Shiku, Hitoshi ^{[2
]}

Matsue, Tomokazu ^{[1
,2
]}

Khademhosseini, Ali ^{[1
,4
,5
,6
,7
,8
]}

机构：

[1] Tohoku Univ, WPI Adv Inst Mat Res, Sendai, Miyagi 9808577, Japan

[2] Tohoku Univ, Grad Sch Environm Studies, Sendai, Miyagi 9808579, Japan

[3] NIMS, RCFM, Tsukuba, Ibaraki 3050047, Japan

[4] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Ctr Biomed Engn, Cambridge, MA 02139 USA

[5] MIT, Harvard MIT Div Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[7] Konkuk Univ, Dept Bioind Technol, Coll Anim Biosci & Technol, Seoul 143701, South Korea

[8] King Abdulaziz Univ, Dept Phys, Fac Sci, Jeddah 21569, Saudi Arabia

来源：

BIOMEDICAL MICRODEVICES | 2017年 / 19卷 / 03期

关键词：

Carbon nanotubes; Cardiac differentiation; Electrical stimulation; Mouse embryoid body; Proliferation; PLURIPOTENT STEM-CELLS; ELECTRICAL-STIMULATION; BODY FORMATION; NANOTECHNOLOGY; PROLIFERATION; SCAFFOLDS; MATRIX; GROWTH; CONDUCTIVITY; STRATEGIES;

D O I：

10.1007/s10544-017-0184-1

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

We embedded carbon nanotubes (CNTs) in mouse embryoid bodies (EBs) for modulating mechanical and electrical cues of the stem cell niche. The CNTs increased the mechanical integrity and electrical conductivity of the EBs. Measured currents for the unmodified EBs (hereafter, EBs) and the EBs-0.25 mg/mL CNTs were 0.79 and 26.3 mA, respectively, at voltage of 5 V. The EBs had a Young's modulus of 20.9 +/- 6.5 kPa, whereas the Young's modulus of the EB-0.1 mg/mL CNTs was 35.2 +/- 5.6 kPa. The EB-CNTs also showed lower proliferation and greater differentiation rates compared with the EBs as determined by the expression of pluripotency genes and the analysis of EB sizes. Interestingly, the cardiac differentiation of the EB-CNTs was significantly greater than that of the EBs, as confirmed by high-throughput gene analysis at day 5 of culture. Applying electrical stimulation to the EB-CNTs specifically enhanced the cardiac differentiation and beating activity of the EBs.

引用

页数：12

共 61 条

[1]

Hybrid hydrogel-aligned carbon nanotube scaffolds to enhance cardiac differentiation of embryoid bodies [J].