Short interfering RNA (siRNA)-mediated RNA interference (RNAi) in human cells

被引：60

作者：

Caplen, NJ

Mousses, S

机构：

[1] NHGRI, Med Genet Branch, NIH, Bethesda, MD 20892 USA

[2] NIH, Canc Drug Dev Lab, Translat Genom Res Inst, Bethesda, MD 20892 USA

来源：

THERAPEUTIC OLIGONUCLEOTIDES: ANTISENSE, RNAI, TRIPLE-HELIX, GENE REPAIR, ENHANCER DECOYS, CPG AND DNA CHIPS | 2003年 / 1002卷

关键词：

siRNAs; short interfering RNAs; RNA interference; RNAi gene silencing;

D O I：

10.1196/annals.1281.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transient gene silencing in mammalian cells can be mediated by double stranded RNA (dsRNAs) molecules of similar to20-25 nucleotides termed short interfering (siRNAs). Naturally occurring siRNAs in lower eukaryotes have characteristic structural elements; however, little is known about what features are critical for an exogenous siRNA to mediate RNAi in mammals. We have recently determined some of the critical parameters that influence the efficiency of siRNA-mediated RNAi in mammalian cells and have been considering the use of RNAi as a functional genomics tool, particularly for high throughput analysis, and the potential use of RNAi as a therapeutic tool.

引用

页码：56 / 62

页数：7

共 63 条

[61] RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals
Zamore, PD
Tuschl, T
Sharp, PA
Bartel, DP
[J]. CELL, 2000, 101 (01) : 25 - 33
[62] RNA interference in human cells is restricted to the cytoplasm
Zeng, Y
Cullen, BR
[J]. RNA, 2002, 8 (07) : 855 - 860
[63] Vector-based RNAi, a novel tool for isoform-specific knock-down of VEGF and anti-angiogenesis gene therapy of cancer
Zhang, L
Yang, N
Mohamed-Hadley, A
Rubin, SC
Coukos, G
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 303 (04) : 1169 - 1178

← 1 2 3 4 5 6 7 →