Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway

被引：276

作者：

Llave, C ^{[1
]}

Kasschau, KD ^{[1
]}

Carrington, JC ^{[1
]}

机构：

[1] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 24期

关键词：

HC-Pro; PTGS suppression; short RNA; methylation;

D O I：

10.1073/pnas.230334397

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Certain plant viruses encode suppressors of posttranscriptional gene silencing (PTGs), an adaptive antiviral defense response that limits virus replication and spread. The tobacco etch potyvirus protein, helper component-proteinase (HC-Pro), suppresses PTGS of silenced transgenes. The effect of HC-Pro on different steps of the silencing pathway was analyzed by using both transient Agrobacterium tumefaciens-based delivery and transgenic systems. HC-Pro inactivated PTGS in plants containing a preexisting silenced beta -glucuronidase (GUs) transgene. PTGS in this system was associated with both small RNA molecules (21-26 nt) corresponding to the 3' proximal region of the transcribed GUS sequence and cytosine methylation of specific sites near the 3' end of the GUs transgene. Introduction of HC-Pro into these plants resulted in loss of PTGs, loss of small RNAs, and partial loss of methylation. These results suggest that HC-Pro targets a PTGs maintenance (as opposed to an initiation or signaling) component at a point that affects accumulation of small RNAs and methylation of genomic DNA.

引用

页码：13401 / 13406

页数：6

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