A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium

被引：47

作者：

Biedendieck, Rebekka

Gamer, Martin

Jaensch, Lothar

Meyer, Steffen

Rohde, Manfred

Deckwer, Wolf-Dieter

Jahn, Dieter

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, D-38106 Braunschweig, Germany

[2] HZI Helmholtz Ctr Infect Res, Dept Cell Biol, D-38124 Braunschweig, Germany

[3] HZI Helmholtz Ctr Infect Res, Dept Microbial Pathogenesis, D-38124 Braunschweig, Germany

[4] Tech Univ Carolo Wilhelmina Braunschweig, TU BCE, D-38124 Braunschweig, Germany

来源：

JOURNAL OF BIOTECHNOLOGY | 2007年 / 132卷 / 04期

关键词：

Bacillus megaterium; sucrose-inducible promoter; recombinant protein production;

D O I：

10.1016/j.jbiotec.2007.07.494

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A sucrose-inducible promoter system (P-sacB) from Bacillus megaterium was identified using a secretome approach. It was successfully employed for the extracellular production of the homologous levansucrase SacB (4252.4 U1(-1)) and the heterologous green fluorescent protein GFP (7.9 mg g(CDW)(-1)). Mutational analysis of B. megaterium P-B allowed the identification of important promoter elements. The sucrose-inducible promoter provides a useful alternative to the established xylose-inducible promoter system (P-xy/A) for recombinant gene expression in B. megaterium. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：426 / 430

页数：5

共 24 条

[1]

[Anonymous], 2001, Anal Biochem

[2] 5'-NONCODING REGION SACR IS THE TARGET OF ALL IDENTIFIED REGULATION AFFECTING THE LEVANSUCRASE GENE IN BACILLUS-SUBTILIS [J].