The Escherichia coli Extracellular Death Factor EDF Induces the Endoribonucleolytic Activities of the Toxins MazF and ChpBK

被引：90

作者：

Belitsky, Maria ^{[1
]}

Avshalom, Haim ^{[1
]}

Erental, Ariel ^{[1
]}

Yelin, Idan ^{[1
]}

Kumar, Sathish ^{[1
]}

London, Nir ^{[1
]}

Sperber, Michal ^{[1
]}

Schueler-Furman, Ora ^{[1
]}

Engelberg-Kulka, Hanna ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Inst Med Res Israel Canada, Dept Microbiol & Mol Genet, IL-91120 Jerusalem, Israel

来源：

MOLECULAR CELL | 2011年 / 41卷 / 06期

基金：

美国国家卫生研究院; 以色列科学基金会;

关键词：

PROGRAMMED CELL-DEATH; ANTITOXIN MODULE; PROTEIN; FAMILY; MAINTENANCE;

D O I：

10.1016/j.molcel.2011.02.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Escherichia coli (E. coli) mazEF is a toxin-antitoxin (TA) stress-induced module that mediates cell death requiring the quorum-sensing pentapeptide NNWNN designated EDF (extracellular death factor). E. coli toxin MazF is a sequence-specific endoribonuclease cleaving single-stranded mRNAs at ACA sequences. E. coli ChpBK, a toxin homologous to MazF, is a sequence-specific endoribonuclease cleaving single-stranded mRNAs at ACA, ACG, and ACU sequences. Here we report that, in vitro, the signaling molecule EDF significantly amplifies the endoribonucleolytic activities of both MazF and ChpBK. EDF also overcomes the inhibitory activity of the antitoxins MazE over the toxin MazF and ChpBI over ChpBK. EDF sequence is important for both functions. Moreover, direct sequence-specific binding of EDF to MazF has been confirmed. Peptide-protein modeling revealed parallel contacts between EDF-MazF and MazE-MazF. These findings are intriguing, since most known quorum-sensing molecules monitor gene expression on the transcriptional level, while EDF monitors posttranscriptionally.

引用

页码：625 / 635

页数：11

共 44 条

[1]

Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis [J].