Characterization of a thy1.2 GFP transgenic mouse reveals a tissue-specific organization of the spinal dorsal horn

被引：7

作者：

Belle, Mino D. ^{[1
]}

Pattison, Edward F. ^{[1
]}

Cheunsuang, Ornsiri ^{[1
,2
]}

Stewart, Anika ^{[1
]}

Kramer, Ina ^{[3
]}

Sigrist, Markus ^{[3
]}

Arber, Silvia ^{[3
]}

Morris, Richard ^{[1
]}

机构：

[1] Univ Liverpool, Dept Vet Preclin Sci, Liverpool L69 7ZJ, Merseyside, England

[2] Chulalongkorn Univ, Dept Vet Anat, Bangkok, Thailand

[3] Univ Basel, Biozentrum, Dept Cell Biol, CH-4056 Basel, Switzerland

来源：

GENESIS | 2007年 / 45卷 / 11期

关键词：

substantia gelatinosa; epidermal sensation; somatosensory; green fluorescent protein; epidermal primary afferents isolectin B4; nonpeptidergic primary afferents; Thy1.2; promoter;

D O I：

10.1002/dvg.20331

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study, transgenic mice in which membrane-linked enhanced green fluorescent protein (mGFP) is expressed from the Thy1.2 promoter were used. In these mice, a subpopulation of small to medium sized DRG neurons double stained for 11134 but not for CGRP Most of the peripheral terminals traversed the dermis and ramify within the epidermis and form superficial terminals. Within the spinal cord, these afferents terminated exclusively within the substantia gelatinosa (SG). A second fibre type in the skin also expressed mGFP, and formed club-shaped endings towards the bases of hairs. Injury to the sciatic nerve resulted in mGFP loss from the SG ipsilateral to the nerve injury, but also in the corresponding region contralaterally. Together, these findings reveal the specificity of connectivity of a defined subpopulation of DRG sensory neurons innervating the epidermis and this will facilitate analysis of their physiological functions. genesis 45:679-688, 2007. (c) 2007 Wiley-Liss, Inc.

引用

页码：679 / 688

页数：10

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