A topoisomerase II-dependent mechanism for resetting replicons at the S-M-phase transition

被引：37

作者：

Cuvier, Olivier ^{[1
]}

Stanojcic, Slavica ^{[1
]}

Lemaitre, Jean-Marc ^{[1
]}

Mechali, Marcel ^{[1
]}

机构：

[1] CNRS, Inst Human Genet, Montpellier 5, France

来源：

GENES & DEVELOPMENT | 2008年 / 22卷 / 07期

关键词：

DNA replication origins; DNA replication foci; checkpoint; ICRF; ORC; DNA combing;

D O I：

10.1101/gad.445108

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Topoisomerase II (topo II) is required for chromosome segregation and for reprogramming replicons. Here, we show that topo II couples DNA replication termination with the clearing of replication complexes for resetting replicons at mitosis. Topo II inhibition impairs completion of DNA replication, accounting for replication protein A (RPA) stabilization onto ssDNA. Topo II inhibition does not affect the caffeine-sensitive ORC1 degradation found upon origin firing, but it impairs the cdk-dependent degradation/chromatin dissociation of an ORC1/2 reservoir at mitosis. Our results show that ORC1 degradation is rescued by Pin1 depletion and that this topo II-dependent clearing of ORC1/2 from chromatin involves the APC.

引用

页码：860 / 865

页数：6

共 34 条

[31] Regulation of replicon size in Xenopus egg extracts [J].