Single amino acid substitution in the murine norovirus capsid protein is sufficient for attenuation in vivo

被引：51

作者：

Bailey, D. ^{[1
]}

Thackray, L. B. ^{[2
]}

Goodfellow, I. G. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Virol, Fac Med, London W2 1PG, England

[2] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO USA

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 15期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JVI.00237-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Murine norovirus (MNV), a prevalent pathogen of laboratory mice, shares many characteristics with human noroviruses. Previous results indicated that passage of MNV1 in the macrophage cell line RAW 264.7 results in attenuation in STAT1-deficient mice (C. E. Wobus, S. M. Karst, L. B. Thackray, K. O. Chang, S. V. Sosnovtsev, G. Belliot, A. Krug, J. M. Mackenzie, K. Y. Green, and H. W. Virgin, PLoS. Biol. 2:e432, 2004). Sequence analysis revealed two amino acid differences between the virulent and attenuated viruses. Using an infectious cDNA clone of the attenuated virus, we demonstrated that a glutamate-to-lysine substitution at position 296 in the capsid protein (VP1) is sufficient to restore virulence in vivo, identifying, for the first time, a virus-encoded molecular determinant of norovirus virulence.

引用

页码：7725 / 7728

页数：4

共 20 条

[11] Genetic diversity and recombination of murine noroviruses in immunocompromised mice [J].

Mueller, B. ;

Klemm, U. ;

Marques, A. Mas ;

Schreier, E. .

ARCHIVES OF VIROLOGY, 2007, 152 (09) :1709-1719

[12] Tissue culture adaptation of foot-and-mouth disease virus selects viruses that bind to heparin and are attenuated in cattle [J].

SaCarvalho, D ;

Rieder, E ;

Baxt, B ;

Rodarte, R ;

Tanuri, A ;

Mason, PW .

JOURNAL OF VIROLOGY, 1997, 71 (07) :5115-5123

[13] Mutations within the P2 domain of norovirus capsid affect binding to human histo-blood group antigens: Evidence for a binding pocket [J].

Tan, M ;

Huang, PW ;

Meller, J ;

Zhong, WM ;

Farkas, T ;

Jiang, X .

JOURNAL OF VIROLOGY, 2003, 77 (23) :12562-12571

[14] Murine noroviruses comprising a single genogroup exhibit biological diversity despite limited sequence divergence [J].

Thackray, Larissa B. ;

Wobus, Christiane E. ;

Chachu, Karen A. ;

Liu, Bo ;

Alegre, Eric R. ;

Henderson, Kenneth S. ;

Kelley, Scott T. ;

Virgin, Herbert W., IV .

JOURNAL OF VIROLOGY, 2007, 81 (19) :10460-10473

[15] Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens [J].

van Loon, AAWM ;

de Haas, N ;

Zeyda, I ;

Mundt, E .

JOURNAL OF GENERAL VIROLOGY, 2002, 83 :121-129

[16] A statistical method for comparing viral growth curves [J].

Wang, Gary P. ;

Bushman, Frederic D. .

JOURNAL OF VIROLOGICAL METHODS, 2006, 135 (01) :118-123

[17] Recovery of infectious murine norovirus using pol II-driven expression of full-length cDNA [J].

Ward, Vernon K. ;

McCormick, Christopher J. ;

Clarke, Ian N. ;

Salim, Omar ;

Wobus, Christiane E. ;

Thackray, Larissa B. ;

Virgin, Herbert W. ;

Lambden, Paul R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (26) :11050-11055

[18] Replication of Norovirus in cell culture reveals a tropism for dendritic cells and macrophages [J].

Wobus, CE ;

Karst, SM ;

Thackray, LB ;

Chang, KO ;

Sosnovtsev, SV ;

Belliot, G ;

Krug, A ;

Mackenzie, JM ;

Green, KY ;

Virgin, HW .

PLOS BIOLOGY, 2004, 2 (12) :2076-2084

[19] Murine norovirus: a model system to study norovirus biology and pathogenesis [J].

Wobus, Christiane E. ;

Thackray, Larissa B. ;

Virgin, Herbert W. .

JOURNAL OF VIROLOGY, 2006, 80 (11) :5104-5112

[20] Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes [J].

Wu, Zhijian ;

Asokan, Aravind ;

Grieger, Joshua C. ;

Govindasamy, Lakshmanan ;

Agbandje-McKenna, Mavis ;

Samulski, R. Jude .

JOURNAL OF VIROLOGY, 2006, 80 (22) :11393-11397

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