Development and characterization of v-myc/v-raf-transformed murine fetal thymocyte cell lines

被引:8
作者
Taubenberger, JK
Reid, AH
Izon, D
Boehme, SA
机构
[1] MONASH UNIV, SCH MED, DEPT PATHOL & IMMUNOL, PRAHRAN, VIC 3181, AUSTRALIA
[2] NIAID, IMMUNOL LAB, NIH, BETHESDA, MD 20892 USA
关键词
D O I
10.1006/cimm.1996.0170
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3(-)CD4(-)CD8(-)) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, were all CD44(+), CD90(+), and were triple negative by surface antigen analysis. The cell. lines, however, were distinguishable by differences in the expression of T cell-associated and T cell-specific genes, The CD3 genes were observed to be discoordinately expressed, in that CD3 gamma chain gene expression was detected in 2 cell lines in the absence of CD3 delta and epsilon expression. Expression of the CD3 gamma chain gene was observed in cell Lines without the expression of other T cell-specific genes or T cell receptor rearrangement and may be one of the earliest T cell-specific genes to be expressed. The transcription factor Ikaros was expressed in all 10 cell lines, whereas the transcription factor TCF1 alpha was expressed only in the 2 most differentiated lines. In 8 cell lines, expression of partial TCR beta and/or TCR alpha transcripts was observed by Northern blot. In several lines, expression of rearranged TCR alpha transcripts in the absence of TCR beta transcripts was demonstrated; however, TCR beta DJ rearrangements were observed by Southern blot in all but 1 of these cell lines, Thus, these cell lines, ordered based on the general pattern of additive gene expression observed, may reflect various stages of triple-negative thymocyte differentiation and provide an in vitro mechanism to elucidate some of the molecular events involved in early thymocyte development. (C) 1996 Academic Press, Inc.
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页码:41 / 47
页数:7
相关论文
共 28 条
[1]  
BALSA E, 1985, NATURE, V318, P667
[2]   CD1 RECOGNITION BY MOUSE NK1(+) T-LYMPHOCYTES [J].
BENDELAC, A ;
LANTZ, O ;
QUIMBY, ME ;
YEWDELL, JW ;
BENNINK, JR ;
BRUTKIEWICZ, RR .
SCIENCE, 1995, 268 (5212) :863-865
[3]   REARRANGEMENT OF T-CELL RECEPTOR BETA-CHAIN GENES DURING T-CELL DEVELOPMENT [J].
BORN, W ;
YAGUE, J ;
PALMER, E ;
KAPPLER, J ;
MARRACK, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (09) :2925-2929
[4]   TRANSCRIPTION FACTORS IN EARLY T-CELL DEVELOPMENT [J].
CLEVERS, HC ;
OOSTERWEGEL, MA ;
GEORGOPOULOS, K .
IMMUNOLOGY TODAY, 1993, 14 (12) :591-596
[5]  
DOSKOW JR, 1992, IMMUNOLOGY, V77, P465
[6]   MOLECULAR AND CELLULAR EVENTS OF T-CELL DEVELOPMENT [J].
FOWLKES, BJ ;
PARDOLL, DM .
ADVANCES IN IMMUNOLOGY, 1989, 44 :207-264
[7]   DEVELOPMENTALLY REGULATED REARRANGEMENT AND EXPRESSION OF GENES ENCODING THE T-CELL RECEPTOR-T3 COMPLEX [J].
FURLEY, AJ ;
MIZUTANI, S ;
WEILBAECHER, K ;
DHALIWAL, HS ;
FORD, AM ;
CHAN, LC ;
MOLGAARD, HV ;
TOYONAGA, B ;
MAK, T ;
VANDENELSEN, P ;
GOLD, D ;
TERHORST, C ;
GREAVES, MF .
CELL, 1986, 46 (01) :75-87
[8]   THE IKAROS GENE IS REQUIRED FOR THE DEVELOPMENT OF ALL LYMPHOID LINEAGES [J].
GEORGOPOULOS, K ;
BIGBY, M ;
WANG, JH ;
MOLNAR, A ;
WU, P ;
WINANDY, S ;
SHARPE, A .
CELL, 1994, 79 (01) :143-156
[9]  
GODFREY DI, 1994, J IMMUNOL, V152, P4783
[10]   REARRANGEMENT AND EXPRESSION OF T-CELL ANTIGEN RECEPTOR AND GAMMA-GENES DURING THYMIC DEVELOPMENT [J].
HAARS, R ;
KRONENBERG, M ;
GALLATIN, WM ;
WEISSMAN, IL ;
OWEN, FL ;
HOOD, L .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (01) :1-24