Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth

被引：325

作者：

Chazaud, B ^{[1
]}

Sonnet, C ^{[1
]}

Lafuste, P ^{[1
]}

Bassez, G ^{[1
]}

Rimaniol, AC ^{[1
]}

Poron, F ^{[1
]}

Authier, FJ ^{[1
]}

Dreyfus, PA ^{[1
]}

Gherardi, RK ^{[1
]}

机构：

[1] Univ Paris 12, Fac Med, Inst Natl Sante & Rech Med, F-94000 Creteil, France

来源：

JOURNAL OF CELL BIOLOGY | 2003年 / 163卷 / 05期

关键词：

skeletal muscle satellite cells; stromal support; muscle regeneration; chemotaxis; myogenesis;

D O I：

10.1083/jcb.200212046

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Once escaped from the quiescence niche, precursor cells interact with stromal components that support their survival, proliferation, and differentiation. We examined interplays between human myogenic precursor cells (mpc) and monocyte/macrophages (MP), the main stromal cell type observed at site of muscle regeneration. mpc selectively and specifically attracted monocytes in vitro after their release from quiescence, chemotaxis declining with differentiation. A DNA macroarray-based strategy identified five chemotactic factors accounting for 77% of chemotaxis: MP-derived chemokine, monocyte chemoattractant protein-1, fractalkine, VEGF, and the urokinase system. MP showed lower constitutive chemotactic activity than mpc, but attracted monocytes much strongly than mpc upon cross-stimulation, suggesting mpc-induced and predominantly MP-supported amplification of monocyte recruitment. Determination of [H-3]thymidine incorporation, oligosomal DNA levels and annexin-V binding showed that MP stimulate mpc proliferation by soluble factors, and rescue mpc from apoptosis by direct contacts. We conclude that once activated, mpc, which are located close by capillaries, initiate monocyte recruitment and interplay with MP to amplify chemotaxis and enhance muscle growth.

引用

页码：1133 / 1143

页数：11