Protease-activated quantum dot probes

被引：177

作者：

Chang, E ^{[1
]}

Miller, JS ^{[1
]}

Sun, JT ^{[1
]}

Yu, WW ^{[1
]}

Colvin, VL ^{[1
]}

Drezek, R ^{[1
]}

West, JL ^{[1
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 334卷 / 04期

基金：

美国国家科学基金会;

关键词：

quantum dot; optical contrast agent; protease imaging; collagenase; fluorescence resonance energy transfer; gold colloid; optical quenching; proteolytic activity; nanoparticle probe; bioimaging;

D O I：

10.1016/j.bbrc.2005.07.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a novel nanoparticulate luminescent probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This construct may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Release of AuNPs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 h of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1317 / 1321

页数：5

共 24 条

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