Sphingosine kinase 1 is required for migration, proliferation and survival of MCF-7 human breast cancer cells

被引：170

作者：

Sarkar, S

Maceyka, M

Hait, NC

Paugh, SW

Sankala, H

Milstien, S

Spiegel, S

机构：

[1] Virginia Commonwealth Univ, Sch Med, Dept Biochem, Richmond, VA 23298 USA

[2] Massey Canc Ctr, Richmond, VA 23298 USA

[3] NIMH, Lab Cellular & Mol Regulat, NIH, Bethesda, MD 20892 USA

来源：

FEBS LETTERS | 2005年 / 579卷 / 24期

关键词：

sphingosine-1-phosphate; sphingosine kinase; epidermal growth factor; MCF-7; cell migration; apoptosis;

D O I：

10.1016/j.febslet.2005.08.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sphingosine-1 -phosphate (S1P) is a potent lysolipid involved in a variety of biological responses important for cancer progression. Therefore, we investigated the role of sphingosine kinase type 1 (SphK1), the enzyme that makes SIP, in the motility, growth, and chemoresistance of MCF-7 breast cancer cells. Epidermal growth factor (EGF), an important growth factor for breast cancer progression, activated and translocated SphK1 to plasma membrane. SphK1 was required for EGF-directed motility. Downregulation of SphK1 in MCF-7 cells reduced EGF- and serum-stimulated growth and enhanced sensitivity to doxorubicin, a potent chemotherapeutic agent. These results suggest that SphK1 may be critical for growth, metastasis and chemoresistance of human breast cancers. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：5313 / 5317

页数：5

相关论文

共 27 条

[1] Involvement of sphingosine in mitochondria-dependent Fas-induced apoptosis of type II Jurkat T cells [J].

Cuvillier, O ;

Edsall, L ;

Spiegel, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (21) :15691-15700

[2] Sphingosine generation, cytochrome c release, and activation of caspase-7 in doxorubicin-induced apoptosis of MCF7 breast adenocarcinoma cells [J].

Cuvillier, O ;

Nava, VE ;

Murthy, SK ;

Edsall, LC ;

Levade, T ;

Milstien, S ;

Spiegel, S .

CELL DEATH AND DIFFERENTIATION, 2001, 8 (02) :162-171

[3] The S1P2 receptor negatively regulates platelet-derived growth factor-induced motility and proliferation [J].

Goparaju, SK ;

Jolly, PS ;

Watterson, KR ;

Bektas, M ;

Alvarez, S ;

Sarkar, S ;

Mel, L ;

Ishii, I ;

Chun, J ;

Milstien, S ;

Spiegel, S .

MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (10) :4237-4249

[4] Role of sphingosine kinase 2 in cell migration toward epidermal growth factor [J].

Hait, NC ;

Sarkar, S ;

Le Stunff, H ;

Mikami, A ;

Maceyka, M ;

Milstien, S ;

Spiegel, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (33) :29462-29469

[5] Role of sphingosine kinase in Ca2+ signalling by epidermal growth factor receptor [J].

Heringdorf, DM ;

Lass, H ;

Kuchar, I ;

Alemany, R ;

Guo, YJ ;

Schmidt, M ;

Jakobs, KH .

FEBS LETTERS, 1999, 461 (03) :217-222

[6] Role of the sphingosine-1-phosphate receptor EDG-1 in PDGF-induced cell motility [J].

Hobson, JP ;

Rosenfeldt, HM ;

Barak, LS ;

Olivera, A ;

Poulton, S ;

Caron, MG ;

Milstien, S ;

Spiegel, S .

SCIENCE, 2001, 291 (5509) :1800-1803

[7] ErbB receptors: Directing key signaling networks throughout life [J].

Holbro, T ;

Hynes, NE .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2004, 44 :195-217

[8] Expression of SphK1 impairs degranulation and motility of RBL-2H3 mast cells by desensitizing S1P receptors [J].

Jolly, PS ;

Bektas, M ;

Watterson, KR ;

Sankala, H ;

Payne, SG ;

Milstien, S ;

Spiegel, S .

BLOOD, 2005, 105 (12) :4736-4742

[9] Sphingosine 1-phosphate activates Erk-1/-2 by transactivating epidermal growth factor receptor in rat-2 cells [J].

Kim, JH ;

Kim, JH ;

Song, WK ;

Kim, JH ;

Chun, JS .

IUBMB LIFE, 2000, 50 (02) :119-124

[10] Molecular cloning and functional characterization of murine sphinogosine kinase [J].

Kohama, T ;

Olivera, A ;

Edsall, L ;

Nagiec, MM ;

Dickson, R ;

Spiegel, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (37) :23722-23728