Development of a quantitative relationship between inhibition percentage and both incubation time and inhibitor concentration for inhibition biosensors - theoretical and practical considerations

被引：58

作者：

Zhang, S ^{[1
]}

Zhao, H ^{[1
]}

John, R ^{[1
]}

机构：

[1] Griffith Univ, Sch Environm & Appl Sci, Gold Coast, Qld 9726, Australia

来源：

BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 9-12期

关键词：

biosensors; microelectrode array; inhibition; paraoxon; pesticides; acetylcholinesterase; choline oxidase;

D O I：

10.1016/S0956-5663(01)00240-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Theoretical and practical insights into the design and development of immobilised enzyme inhibition biosensors are reported. A general mathematical expression relating the percent of enzyme inhibition (i.e. the analytical signal) to both the inhibitor concentration and the incubation time is presented. The relevant physical, chemical and biochemical parameters required by the model are developed and discussed in terms of the inhibition of acetylcholinesterase by the organophosphorous pesticide, paraoxon. A second enzyme, choline oxidase and an amperometric transducer are used to facilitate the determination acetylcholinesterase inhibitor. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：1119 / 1126

页数：8