Recombinant protein production in yeasts

被引：141

作者：

Porro, D

Sauer, M

Branduardi, P

Mattanovich, D

机构：

[1] Univ Milano Bicocca, Dept Biosci & Biotechnol, I-20126 Milan, Italy

[2] Univ Nat Resources & Appl Life Sci, BOKU, Inst Appl Microbiol, A-1190 Vienna, Austria

来源：

MOLECULAR BIOTECHNOLOGY | 2005年 / 31卷 / 03期

关键词：

yeasts; heterologous proteins; expression; industrial biotechnology;

D O I：

10.1385/MB:31:3:245

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow the production of a wide range of peptides, proteins, and biochemicals from naturally nonproducing cells. These technologies, now approx 25 yr old, have become one of the most important technologies developed in the twentieth century. Pharmaceutical products and industrial enzymes were the first biotech products on the world market made by means of rDNA. Despite important advances in rDNA applications in mammalian cells, yeasts still represent attractive hosts for the production of heterologous proteins. In this review we summarize advantages and limitations of the main and most promising yeast hosts.

引用

收藏

页码：245 / 259

页数：15

相关论文

共 75 条

[1]

BINDER M, 1991, EUR J CELL BIOL, V54, P305

[2] PHYSIOLOGICAL APPROACH TO HETEROLOGOUS HUMAN SERUM-ALBUMIN PRODUCTION BY KLUYVEROMYCES-LACTIS IN CHEMOSTAT CULTURE [J].

BLONDEAU, K ;

BOZE, H ;

JUNG, G ;

MOULIN, G ;

GALZY, P .

YEAST, 1994, 10 (10) :1297-1303

[3] Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris:: production of complex humanized glycoproteins with terminal galactose [J].

Bobrowicz, P ;

Davidson, RC ;

Li, HJ ;

Potgieter, TI ;

Nett, JH ;

Hamilton, SR ;

Stadheim, TA ;

Miele, RG ;

Bobrowicz, B ;

Mitchell, T ;

Rausch, S ;

Renfer, E ;

Wildt, S .

GLYCOBIOLOGY, 2004, 14 (09) :757-766

[4]

BRAMBILLA L, 2000, Patent No. 0041477

[5] The yeast Zygosaccharomyces bailii:: a new host for heterologous protein production, secretion and for metabolic engineering applications [J].

Branduardi, P ;

Valli, M ;

Brambilla, L ;

Sauer, M ;

Alberghina, L ;

Porro, D .

FEMS YEAST RESEARCH, 2004, 4 (4-5) :493-504

[6] YEAST SYSTEMS FOR THE COMMERCIAL PRODUCTION OF HETEROLOGOUS PROTEINS [J].

BUCKHOLZ, RG ;

GLEESON, MAG .

BIO-TECHNOLOGY, 1991, 9 (11) :1067-1072

[7] TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION [J].

BURGERS, PMJ ;

PERCIVAL, KJ .

ANALYTICAL BIOCHEMISTRY, 1987, 163 (02) :391-397

[8] Degradation of proteins from the ER of S-cerevisiae requires an intact unfolded protein response pathway [J].

Casagrande, R ;

Stern, P ;

Diehn, M ;

Shamu, C ;

Osario, M ;

Zúñiga, M ;

Brown, PO ;

Ploegh, H .

MOLECULAR CELL, 2000, 5 (04) :729-735

[9]

Cereghino JL, 2000, FEMS MICROBIOL REV, V24, P45, DOI 10.1016/S0168-6445(99)00029-7

[10] Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris [J].

Choi, BK ;

Bobrowicz, P ;

Davidson, RC ;

Hamilton, SR ;

Kung, DH ;

Li, HJ ;

Miele, RG ;

Nett, JH ;

Wildt, S ;

Gerngross, TU .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (09) :5022-5027

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