CD4+ Th1 and CD8+ type 1 cytotoxic T cells both play a crucial role in the full development of contact hypersensitivity

被引:180
作者
Wang, BH
Fujisawa, H
Zhuang, LH
Freed, I
Howell, BG
Shahid, S
Shivji, GM
Mak, TW
Sauder, DN
机构
[1] Univ Toronto, Sunnybrook & Womens Coll, Hlth Sci Ctr, Div Dermatol, Toronto, ON M4N 3M5, Canada
[2] Univ Toronto, Dept Med Biophys, Amgen Inst, Toronto, ON, Canada
[3] Univ Toronto, Dept Immunol, Toronto, ON, Canada
[4] Univ Tsukuba, Dept Dermatol, Tsukuba, Ibaraki 305, Japan
关键词
D O I
10.4049/jimmunol.165.12.6783
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The role of CD4(+) vs CD8(+) T cells in contact hypersensitivity (CHS) remains controversial. In this study, we used gene knockout (KO) mice deficient in CD4(+) or CD8(+) T cells to directly address this issue. Mice lacking either CD4(+) or CD8(+) T cells demonstrated depressed CHS responses to dinitrofluorobenzene and oxazolone compared with wild-type C57BL16 mice. The depression of CHS was more significant in CD8 KO mice than in CD4 KO mice, Furthermore, in vivo depletion of either CD8(+) T cells from CD4 KO mice or CD4(+) T cells from CD8 KO mice virtually abolished CHS responses. Lymph node cells (LNCs) from hapten-sensitized CD4 and CD8 KO mice showed a decreased capacity for transferring CHS. in vitro depletion of either CD4(+) T cells from CD8 KO LNCs or CD8(+) T cells from CD4 KO LNCs resulted in a complete loss of CHS transfer. LNCs from CD4 and CD8 KO mice produced significant amounts of IFN-gamma, indicating that both CD4(+) and CD8(+) T cells are able to secrete IFN-gamma, LNCs from CD8, but not CD4, KO mice were able to produce IL-4 and IL-10, suggesting that IL-4 and IL-10 are mainly derived from CD4(+) T cells. Intracellular cytokine staining of LNCs confirmed that IFN-gamma -positive cells consisted of CD4(+) (Th1) and CD8+ (type 1 cytotoxic T) T cells, whereas IL-10-positive cells were exclusively CD4(+) (Th2) T cells. Collectively, these results suggest that both CD4(+) Th1 and CD8(+) type 1 cytotoxic T cells are crucial effector cells in CHS responses to dinitrofluorobenzene and oxazolone in C57BL/6 mice.
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收藏
页码:6783 / 6790
页数:8
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