X-ray contact microscopy system for spectromicroscopy of biological specimens

被引：9

作者：

Ito, A

Shinohara, K

Mizukami, Y

Nakano, H

Yada, K

Uehara, T

Honda, T

机构：

[1] Tokai Univ, Dept Nucl Engn, Sch Engn, Hiratsuka, Kanagawa 25912, Japan

[2] Univ Tokyo, Fac Med, Radiat Res Inst, Bunkyo Ku, Tokyo 113, Japan

[3] Chiba Univ, Fac Engn, Dept Image Sci, Inage Ku, Chiba 263, Japan

[4] Tokyo Metropolitan Inst Med Sci, Dept Radiat Res, Bunkyo Ku, Tokyo 113, Japan

[5] Aomori Publ Coll, Goshizawa, Aomori 030, Japan

来源：

JOURNAL OF SYNCHROTRON RADIATION | 1998年 / 5卷

关键词：

spectromicroscopy; XANES; element analysis; mammalian cells;

D O I：

10.1107/S0909049597018931

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

An X-ray contact microscopy system has been developed for the study of molecular and elemental distributions in biological specimens based on X-ray absorption characteristics. The system consists of a chamber for measuring XANES (X-ray absorption near-edge structure) of biomolecules, and a contact microscopy system far dried specimens with an electronic zooming tube. With this system the elemental distribution of carbon, nitrogen, oxygen, Ca and Fe in an HeLa cell has been studied, and the DNA-related image using a peak from the XANES profile of the DNA at the K-absorption edge of phosphorus has been obtained.

引用

页码：1099 / 1101

页数：3