Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation

被引:28
作者
Danesh, Ali [2 ,3 ]
Cameron, Cheryl M. [2 ]
Leon, Alberto J. [1 ]
Ran, Longsi [2 ]
Xu, Luoling [2 ]
Fang, Yuan [2 ,3 ]
Kelvin, Alyson A. [4 ,7 ]
Rowe, Thomas [2 ]
Chen, Honglin [1 ]
Guan, Yi [1 ]
Jonsson, Colleen B. [5 ]
Cameron, Mark J. [2 ]
Kelvin, David J. [1 ,2 ,6 ]
机构
[1] Shantou Univ, Coll Med, Int Inst Infect & Immun, Shantou 515031, Guangdong, Peoples R China
[2] Univ Hlth Network, Toronto Gen Res Inst, Div Expt Therapeut, Toronto, ON M5G 1L7, Canada
[3] Univ Toronto, Fac Med, Dept Immunol, Toronto, ON M5G 1L7, Canada
[4] MaRS Ctr, IDR, Toronto, ON M5G 1L7, Canada
[5] Ctr Predict Med, Louisville, KY 40222 USA
[6] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy
[7] Sardinia Res & Dev Srl, SaRD, Porto Conte Ric Res Ctr, I-07041 Alghero, SS, Italy
关键词
Ferret; Gene expression; SARS; Interferon; ACUTE RESPIRATORY SYNDROME; SIGNALING NETWORK; IMMUNE-RESPONSES; UNITED-STATES; IN-VIVO; INFLUENZA; INNATE; VIRUS; TRANSCRIPTION; EMERGENCE;
D O I
10.1016/j.virol.2010.10.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Type I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-alpha 2b and during SARS-CoV infection. In vivo experiments revealed that IFN-alpha 2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:102 / 112
页数:11
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