Impedimetric characterization of the changes produced in the electrode-solution interface by bacterial attachment

被引：28

作者：

Munoz-Berbel, Xavier ^{[1
]}

Vigues, Nuria ^{[2
]}

Mas, Jordi ^{[2
]}

Toby, A. ^{[3
]}

Jenkins, A. ^{[3
]}

Munoz, Francisco J. ^{[1
]}

机构：

[1] CSIC, IMB, Ctr Nacl Microelect, Barcelona 08193, Spain

[2] Univ Autonoma Barcelona, Grp Microbiol Ambiebtal, E-08193 Barcelona, Spain

[3] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2007年 / 9卷 / 11期

关键词：

impedance spectroscopy; bacterial attachment; fluorescence microscopy; bacterial quantification; platinum chips;

D O I：

10.1016/j.elecom.2007.08.011

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

This paper describes a new method for measuring the attachment of bacteria, specifically Escherichia coli on platinum electrodes using impedance spectroscopy. Impedance spectroscopy measurements showed that the double layer capacitance of the electrode was very sensitive both to the concentration of bacteria in the solution and to the attachment time. Impedance measurements of E. coli were compared with classical measurements of bacterial attachment on identical electrodes such as staining/microscopy and bacterial removal by sonication and plating onto agar. The relationship between the measured impedance of the electrode during attachment and the biophysical processes involved is discussed. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：2654 / 2660

页数：7