Cloning and characterization of the human galanin GALR2 receptor

被引：41

作者：

Borowsky, B

Walker, MW

Huang, LY

Jones, KA

Smith, KE

Bard, J

Branchek, TA

Gerald, C

机构：

[1] Synapt Pharmaceut Corp, Dept Biol Mol, Paramus, NJ 07652 USA

[2] Synapt Pharmaceut Corp, Dept Pharmacol, Paramus, NJ 07652 USA

来源：

PEPTIDES | 1998年 / 19卷 / 10期

关键词：

galanin; receptor; G protein-coupled receptor; reverse transcriptase polymerase chain reaction (PCR); phosphatidyl inositol hydrolysis;

D O I：

10.1016/S0196-9781(98)00133-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the molecular cloning and characterization of the human galanin receptor, hGALR2. hGALR2 shares 85%, 39%, and 57% amino acid identities to rGALR2, hGALR1, and hGALR3, respectively, hGALR2, along with rGALR2, can be distinguished from the other cloned galanin receptors by a tolerance for both N-terminal extension and C-terminal deletion of galanin, as well as by a primary signaling mechanism involving phosphatidyl inositol hydrolysis and calcium mobilization. By RT-PCR, GALR2 mRNA was abundant in human hippocampus, hypothalamus, heart, kidney, Liver, and small intestine. A weak GALR2 mRNA signal was detected in human retina, and no signal was detected in cerebral cortex, lung, spleen, stomach, or pituitary.

引用

页码：1771 / 1781

页数：11

共 35 条

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