New genotype-phenotype linkages for directed evolution of functional proteins

被引：97

作者：

Leemhuis, H

Stein, V

Griffiths, AD

Hollfelder, F ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[2] Univ Strasbourg, ISIS, F-67083 Strasbourg, France

[3] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2005年 / 15卷 / 04期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

D O I：

10.1016/j.sbi.2005.07.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The key practical consideration in directed evolution of functional biomolecules is the linkage of genotype and phenotype. In vitro selections offer the potential to select from libraries with up to 10(10)-10(14) members, with fewer constraints than current cell-based selections. New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis.

引用

收藏

页码：472 / 478

页数：7

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