The two component adjuvant IC31® potentiates the protective immunity induced by a dengue 2 recombinant fusion protein in mice

被引：16

作者：

Bernardo, Lidice ^{[1
]}

Pavon, Alequis ^{[1
]}

Hermida, Lisset ^{[2
]}

Gil, Lazaro ^{[2
]}

Valdes, Iris ^{[2
]}

Cabezas, Sheila ^{[1
]}

Linares, Ramon ^{[1
]}

Alvarez, Mayling ^{[1
]}

Silva, Ricardo ^{[2
]}

Guillen, Gerardo ^{[2
]}

Nagy, Eszter ^{[3
]}

Schlick, Petra ^{[3
]}

Guzman, Maria G. ^{[1
]}

机构：

[1] PAHO WHO Collaborating Ctr Study Dengue & Its Vec, Dept Virol, Pedro Kouri Trop Med Inst, Havana, Cuba

[2] Ctr Genet Engn & Biotechnol, Havana 10600, Cuba

[3] Intercell AG, A-1030 Vienna, Austria

来源：

VACCINE | 2011年 / 29卷 / 25期

关键词：

Dengue; Domain III; IC31 (R) adjuvant; VIRUS ENVELOPE GLYCOPROTEIN; ANTIMYCOBACTERIAL T-CELL; DOMAIN-III; MONOCLONAL-ANTIBODIES; NEUTRALIZING ANTIBODIES; VACCINE ADJUVANT; DENDRITIC CELLS; P64K PROTEIN; RESPONSES; INFECTION;

D O I：

10.1016/j.vaccine.2011.03.040

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Here we evaluated the suitability of the synthetic adjuvant IC31 (R) to potentiate the protective capacity of PD5 protein (domain III of the envelope protein of dengue 2 virus fused to the carrier protein P64k). Unlike Alum, PD5 mixed with IC31 (R) induced complete protection against virus challenge in mice and increased IFN-gamma secretion after in vitro re-stimulation. The induced antibody response was highly specific to the homologous serotype and showed both IgG1 and IgG2a subtypes. IC31 (R) is a promising adjuvant for PD5 recombinant protein based vaccination against dengue. Future work should address the suitability of PD5/IC31 (R) formulations in non-human primate models. (C) 2011 Published by Elsevier Ltd.

引用

页码：4256 / 4263

页数：8

共 48 条

[1] Protection and Polyfunctional T Cells Induced by Ag85B-TB10.4/IC31® against Mycobacterium tuberculosis Is Highly Dependent on the Antigen Dose [J].

Aagaard, Claus ;

Hoang, Truc Thi Kim Thanh ;

Izzo, Angelo ;

Billeskov, Rolf ;

Troudt, JoLynn ;

Arnett, Kim ;

Keyser, Andrew ;

Elvang, Tara ;

Andersen, Peter ;

Dietrich, Jes .

PLOS ONE, 2009, 4 (06)

[2] Protective immunity to tuberculosis with Ag85B-ESAT-6 in a synthetic cationic adjuvant system IC31 [J].

Agger, Else Marie ;

Rosenkrands, Ida ;

Olsen, Anja Weinreich ;

Hatch, Graham ;

Williams, Ann ;

Kritsch, Constantia ;

Lingnau, Karen ;

von Gabain, Alexander ;

Andersen, Claire Swetman ;

Korsholm, Karen Smith ;

Andersen, Peter .

VACCINE, 2006, 24 (26) :5452-5460

[3]

Alvarez M, 2005, DENGUE B, V29, P1

[4] Immunogenicity and protective efficacy of a recombinant fusion protein containing the domain III of the dengue 1 envelope protein in non-human primates [J].

Bernard, Lidice ;

Izquierdo, Alienys ;

Alvarez, Mayling ;

Rosario, Delfina ;

Prado, Irina ;

Lopez, Carlos ;

Martinez, Rafael ;

Castro, Jorge ;

Santana, Ernidalys ;

Hermida, Lisset ;

Guillen, Gerardo ;

Guzman, Maria G. .

ANTIVIRAL RESEARCH, 2008, 80 (02) :194-199

[5] Structures of immature flavivirus particles [J].

Zhang, Y ;

Corver, J ;

Chipman, PR ;

Zhang, W ;

Pletnev, SV ;

Sedlak, D ;

Baker, TS ;

Strauss, JH ;

Kuhn, RJ ;

Rossmann, MG .

EMBO JOURNAL, 2003, 22 (11) :2604-2613

[6] TECHNIQUES FOR HEMAGGLUTINATION AND HEMAGGLUTINATION-INHIBITION WITH ARTHROPOD-BORNE VIRUSES [J].

CLARKE, DH ;

CASALS, J .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1958, 7 (05) :561-573

[7] Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells [J].

Crill, WD ;

Roehrig, JT .

JOURNAL OF VIROLOGY, 2001, 75 (16) :7769-7773

[8] The artificial antimicrobial peptide KLKLLLLLKLK induces predominantly a TH2-type immune response to co-injected antigens [J].

Fritz, JH ;

Brunner, S ;

Birnstiel, ML ;

Buschle, M ;

Gabain, AV ;

Mattner, F ;

Zauner, W .

VACCINE, 2004, 22 (25-26) :3274-3284

[9] The Cellular Immune Response Plays an Important Role in Protecting Against Dengue Virus in the Mouse Encephalitis Model [J].

Gil, Lazaro ;

Lopez, Carlos ;

Blanco, Aracelys ;

Lazo, Laura ;

Martin, Jorge ;

Valdes, Iris ;

Romero, Yaremis ;

Figueroa, Yassel ;

Guillen, Gerardo ;

Hermida, Lisset .

VIRAL IMMUNOLOGY, 2009, 22 (01) :23-29

[10] Towards a dengue vaccine: Progress to date and remaining challenges [J].

Guy, Bruno ;

Almond, Jeffrey W. .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2008, 31 (2-3) :239-252

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