Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose

被引：157

作者：

Gusakov, Alexander V. ^{[1
]}

Salanovich, Tatyana N.

Antonov, Alexey I.

Ustinov, Boris B.

Okunev, Oleg N.

Burlingame, Richard

Emalfarb, Mark

Baez, Marco

Sinitsyn, Arkady P.

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia

[2] Russian Acad Sci, Inst Biochem & Physiol Microorganisms, Pushchino 142292, Moscow Region, Russia

[3] Dyadic Int Inc, Jupiter, FL 33477 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2007年 / 97卷 / 05期

关键词：

cellobiohydrolase; cellulase; Chrysosporium lucknowense; endoglucanase; Penicillium sp; Trichoderma reesei;

D O I：

10.1002/bit.21329

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An extremely highly active cellobiolrydrolase (CBH Ilb or Ce16B) was isolated from Chrysosporium lucknowense UV18-25 culture filtrate. The CBH IIb demonstrated the highest ability for a deep degradation of crystalline cellulose amongst a few cellobiohydrolases tested, including C. lucknowense CBH la, Ib, IIa, and Trichoderma reesei CBH I and II. Using purified C. lucknowense enzymes (CBH la, Ib, and IIb; endoglucanases II and V; beta-glucosidase, xylanase II), artificial multienzyme mixtures were reconstituted, displaying an extremely high performance in a conversion of different cellulosic substrates (Avicel, cotton, pretreated Douglas fir wood) to glucose. These mixtures were much or notably more effective in hydrolysis of the cellulosic substrates than the crude multienzyme C. lucknowense preparation and other crude cellulase samples produced by T. reesei and Penicillium verruculosum. Highly active cellulases are a key factor in bioconversion of plant lignocellulosic biomass to ethanol as an alternative to fossil fuels.

引用

页码：1028 / 1038

页数：11

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